2-Deoxy-D-glucose(2-DG)（葡萄糖模拟物）是一个竞争性糖酵解的抑制剂[1 - 3]。细胞毒性试验中对KIT-阳性细胞株GIST882和GIST430的IC50值分别为0.5 μM和2.5 μM[4]。

糖酵解是ATP-产生的一个子系统[5]。

如果2-DG/葡萄糖的生理相关比率是0.8，在包含25 mmol/L葡萄糖的DMEM中生长的FaDu细胞中葡萄糖代谢将会被抑制。相比于对照细胞，2-DG处理导致总谷胱甘肽含量减少了30%到40%，细胞被杀死了32%[2]。2-DG以浓度依赖式地抑制PEDV传播，诱导Vero细胞内质网应激反应。2-DG没有明显地影响病毒数。但进一步的研究表明，2-DG降低病毒蛋白质翻译，这意味着在病毒感染的早期阶段，2-DG可能影响病毒的复制。此外，在病毒感染的早期阶段2-DG增加了CD13表达水平[6]。

在体内，2-DG在人骨肉瘤和非小细胞肺癌中增加阿霉素和紫杉醇的疗效，可阻碍肿瘤的生长，延长存活时间[3]。在金头鲷中，2-DG显著地增加血糖水平，引起非统计性显著降低头肾白细胞呼吸活性。头肾白细胞血清中过氧化物酶活性与对照相比无显著差异[7]。

参考文献：
[1]. John Laszlo, William R. Harlan, Robert F. Klein, et al. The Effect of 2-Deoxy-D-Glucose Infusions on Lipid and Carbohydrate Metabolism in Man. J Clin Invest., 1961, 40(1):171-176.
[2]. Andrean L. Simons, Iman M. Ahmad, David M. Mattson, et al. 2-Deoxy-D-Glucose Combined with Cisplatin Enhances Cytotoxicity via Metabolic Oxidative Stress in Human Head and NeckCancer Cells. Cancer Research, 2007, 67(7): 3364-3371.
[3]. Gregory Maschek, Niramol Savaraj, Waldemar Priebe, et al. 2-Deoxy-D-glucose Increases the Efficacy of Adriamycin and Paclitaxel in Human Osteosarcoma and Non-Small Cell Lung Cancers In Vivo. Cancer Research, 2004, 64:31-34.
[4]. Thomas Mühlenberg, Susanne Grunewald, Jürgen Treckmann, et al. Inhibition of KIT-Glycosylation by 2-Deoxyglucose Abrogates KIT-Signaling and Combination with ABT-263 Synergistically Induces Apoptosis in Gastrointestinal Stromal Tumor. PLOS ONE, 2015, 10(3):e0120531.
[5]. R. J. Connett, C. R. Honig, T. E. Gayeski, et al. Defining hypoxia: a systems view of VO2, glycolysis, energetics, and intracellular PO2. Journal of Applied Physiology, 1990, 68(3): 833-842.
[6]. Yue Wang, Jia-rong Li, Ming-xia Sun, et al. Triggering unfolded protein response by 2-Deoxy-D-glucose inhibits porcine epidemic diarrhea virus propagation. Antiviral Research, 2014, 106: 33–41.
[7]. F.A. Guardiola, R. Cerezuela, J. Meseguer, et al. Effects of 2-deoxy-D-glucose on the immune system of seabream (Sparus aurata L.). Fish & Shellfish Immunology, 2011, 30: 592-599.