Sildenafil mesylate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50为3.5 nM。
Sildenafil mesylate是一种环鸟苷酸（cGMP）特异性磷酸二酯酶5型（PDE5）抑制剂。
PDE5是海绵体中主要的磷酸二酯酶。PDE5的催化位点降解cGMP，并且PDE5抑制剂可增强cGMP的内源性增加。
体外：Sildenafil mesylate已被鉴定为一种可逆的和选择性的强效PDE5抑制剂。在器官浴槽中，Sildenafil增强硝普钠或透壁电刺激诱导的预收缩海绵体肌带的松弛。在硝普钠处理的平滑肌细胞培养物中，Sildenafil还增加细胞内cGMP浓度[1]。
体内：在麻醉犬中，Sildenafil可增强盆腔神经刺激后的勃起功能，测得海绵体内压力增加[1]。
临床试验：已进行10项随机对照试验。剂量优化引起> 60％的性交尝试，在49％的男性中取得成功。30％的男性出现Sildenafil治疗相关不良事件，而安慰剂组为11％。Sildenafil剂量优化表现出等同于最高固定剂量的功效，以及等同于最低固定剂量的不良事件[2]。
参考文献：
[1] Nehra A,Colreavy F,Khandheria BK,Chandrasekaran K. Sildenafil citrate, a selective phosphodiesterase type 5 inhibitor: urologic and cardiovascular implications. World J Urol.2001 Feb;19(1):40-5.
[2] Moore RA,Edwards JE,McQuay HJ. Sildenafil (Viagra) for male erectile dysfunction: a meta-analysis of clinical trial reports. BMC Urol.2002 May 22;2:6.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 570.68 |
| Cas No. | 1308285-21-3 |
| Formula | C23H34N6O7S2 |
| Solubility | ≥57.1 mg/mL in DMSO; insoluble in EtOH; ≥25.95 mg/mL in H2O |
| Chemical Name | 5-(2-ethoxy-5-((4-methylpiperazin-1-yl)sulfonyl)phenyl)-1-methyl-3-propyl-1H-pyrazolo[4,3-d]pyrimidin-7(6H)-one methanesulfonate |
| SDF | Download SDF |
| Canonical SMILES | CCCC(C(N=C(N1)C2=C(OCC)C=CC(S(N3CCN(CC3)C)(=O)=O)=C2)=C4C1=O)=NN4C.CS(O)(=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
