SB-3CT
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SB-3CT是有效的明胶酶选择性抑制剂,对MMP-2和MMP-9的Ki值分别为13.9和600 nM.
明胶酶A(MMP-2)和B(MMP-9)属于基质金属蛋白酶家族,通过水解细胞外基质参与肿瘤转移和血管生成[1].
SB-3CT是一种有效的明胶酶选择性抑制剂.SB-3CT是一种新的基于机制的抑制剂,可直接结合MMP-2的催化锌离子[1].SB-3CT可抑制纯化的小鼠MMP-9,Ki值为120 nM[2].
在携带T细胞淋巴瘤L-CI.5s细胞的小鼠中,SB-3CT在50 mg/kg/d下可以剂量依赖的方式减少肝转移的数量,并可减少大于70%的肿瘤集落数量.SB-3CT(50 mg/kg/d)也可显著减小集落大小和肝脏PCNA阳性肿瘤细胞数量.这些结果表明SB-3CT可有效抑制肿瘤细胞溢出,并具有抗增殖效应.SB-3CT可通过抑制MMP-2和MMP-9,完全抑制MMP介导的溶明胶活性.并且,SB-3CT可增加存活率[2].在短暂局灶性脑缺血小鼠模型中,SB-3CT可抑制MMP-9介导的层粘连蛋白裂解,而后拯救神经元免于凋亡[2].
参考文献：
[1]. Kleifeld O, Kotra LP, Gervasi DC, et al. X-ray absorption studies of human matrix metalloproteinase-2 (MMP-2) bound to a highly selective mechanism-based inhibitor. comparison with the latent and active forms of the enzyme. J Biol Chem, 2001, 276(20): 17125-17131.
[2]. Krüger A, Arlt MJ, Gerg M, et al. Antimetastatic activity of a novel mechanism-based gelatinase inhibitor. Cancer Res, 2005, 65(9): 3523-3526.
[3]. Gu Z, Cui J, Brown S, et al. A highly specific inhibitor of matrix metalloproteinase-9 rescues laminin from proteolysis and neurons from apoptosis in transient focal cerebral ischemia. J Neurosci, 2005, 25(27): 6401-6408.
| Physical Appearance | A solid |
| Storage | Desiccate at -20°C |
| M.Wt | 306.40 |
| Cas No. | 292605-14-2 |
| Formula | C15H14O3S2 |
| Solubility | ≥30.6 mg/mL in DMSO; insoluble in H2O; ≥2.43 mg/mL in EtOH |
| Chemical Name | 2-(((4-phenoxyphenyl)sulfonyl)methyl)thiirane |
| SDF | Download SDF |
| Canonical SMILES | O=S(CC1CS1)(C2=CC=C(OC3=CC=CC=C3)C=C2)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
