S-Adenosylhomocysteine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
S-adenosylhomocysteine是代谢途径中的一种氨基酸衍生物,是合成腺苷和半胱氨酸的中间体。
体外:先前研究发现,胱硫醚β-合酶(CBS)缺陷酵母可以比野生型酵母转运显著更多的同型半胱氨酸进入培养基,具有升高的同型半胱氨酸和S-adenosylhomocysteine (SAH)。此外,25 μM的SAH抑制CBS缺陷型酵母的生长,而对野生型酵母没有影响。此外,细胞内S-adenosylmethionine (SAM)和SAH的测量结果表明,与野生型酵母相比,CBS缺陷型酵母具有降低的SAM/SAH比率。同时,SAH在CBS缺陷型酵母中的生长抑制可以通过向培养基中加入SAM来逆转,表明对于细胞生长至关重要的是SAM/SAH比率而非绝对水平[1]。
体内:研究发现SAH的组织分布在不同性别大鼠中相似,并且仅随年龄轻微改变。在检查的所有组织中,SAH水解酶的比活性大大超过甲硫氨酸腺苷转移酶的比活性,S-adenosylmethionine浓度高于S-adenosylhomocysteine。然而,肝S-adenosylmethionine/SAH比率取决于食物供应和动物的年龄。甲硫氨酸腹腔注射导致S-adenosylmethionine和SAH肝脏浓度的深度但短暂的增加[2]。
临床试验:到目前为止,S-adenosylhomocysteine尚未临床使用。
参考文献:
[1] Christopher SA,Melnyk S,James SJ,Kruger WD. S-adenosylhomocysteine, but not homocysteine, is toxic to yeast lacking cystathionine beta-synthase. Mol Genet Metab.2002 Apr;75(4):335-43.
[2] Eloranta TO. Tissue distribution of S-adenosylmethionine and S-adenosylhomocysteine in the rat. Effect of age, sex and methionine administration on the metabolism of S-adenosylmethionine, S-adenosylhomocysteine and polyamines. Biochem J.1977 Sep 15;166(3):521-9.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 384.41 |
Cas No. | 979-92-0 |
Formula | C14H20N6O5S |
Solubility | insoluble in EtOH; ≥45.3 mg/mL in H2O with gentle warming; ≥8.56 mg/mL in DMSO with gentle warming and ultrasonic |
Chemical Name | (S)-2-amino-4-((((2S,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl)thio)butanoic acid |
SDF | Download SDF |
Canonical SMILES | O[C@@H]1[C@@H]([C@H](O[C@H]1N2C3=C(N=C2)C(N)=NC=N3)CSCC[C@@H](C(O)=O)N)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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