Cyclic AMP
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 500mg | ￥681.00 | 现货 | |
| 1g | ￥1090.00 | 现货 | |
| 5g | ￥3272.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
Cyclic adenosine 3’, 5’-monophosphate (cAMP) was the first second messenger to be identified, with the IC50 of LPA- or EGF-stimulated ERK1 was 0.1-0.2 Mm [1].
Ras is a common target for growth factors including epidermal growth factor (EGF) and lysophosphatidic acid (LPA), which activate the Raf-MEK-ERK pathway. Raf is likely to be a link between Ras and ERK cascade, of which Raf-1activates MEK in vitro.
Quiescent Rat1 cells were incubated with 1 Mm dibutyryl cAMP for 10 min before stimulation with LPA or EGF, ERK activation was completely inhibited by protein immunoblots. The ability of the phorbol ester PMA to activate MAP kinase was also inhibited by dibutyryl cAMP [1].
Reference:
[1] Cook S J, McCormick F. Inhibition by cAMP of Ras-Dependent Activation of Raf [J]. Science. 1993, 262(5136):1069-1072.
产品性质
| CAS号 | 60-92-4 |
| 分子式 | C10H12N5O6P |
| 分子量 | 329.21 |
| 化学名称 | (4aR,6R,7R,7aS)-6-(6-amino-9H-purin-9-yl)-2,7-dihydroxytetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinine 2-oxide |
| 溶解度 | insoluble in DMSO; insoluble in EtOH; ≥16.45 mg/mL in H2O |
| SMILES | O[C@H]1[C@H](N2C=NC3=C2N=CN=C3N)O[C@@]4([H])[C@@]1([H])OP(OC4)(O)=O |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
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