PRL-3 Inhibitor
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 900 nM: blocks human phosphatase of regenerating liver 3 (PRL-3) in vitro.
PRL-3 inhibitor, a cell-permeable benzylidene rhodamine compound, potently dampens PRL-3 and inhibits other phosphatases with minimal activity. It has been demonstrated that PRL-3 inhibitor can be used as therapeutics for the treatment of cancer. PRL-3 is a member of the regenerating liver family tyrosine phosphatases and plays vital roles in cell proliferation, migration, invasion, and motility, and thus leads to cancer metastasis.
In vitro: PRL-3 inhibitor, in a dose-dependent fashion, blocked the dephosphorylation of DiFMUP by PRL-3 and strongly suppressed the activity of PRL-3 phosphatase in PRL-3 overexpressing DLD-1 colon tumor cells [DLD-1 (PRL-3)]. Also, PRL-3 inhibitor dose-dependently blocked the migration of DLD-1 (PRL-3) cells, however, it did not inhibit the proliferation of DLD-1(PRL-3) cells, suggesting that PRL-3 inhibitor significantly blocked cell immigration and invasion without cytotoxicity [1].
In vivo: Up to now, in vivo study of PRL-3 Inhibitor is still in the development stage.
Reference:
[1]. Min, G., Lee, S., Kim, H., Han, Y., Lee, R., & Jeong, D. et al. Rhodanine-based PRL-3 inhibitors blocked the migration and invasion of metastatic cancer cells. Bioorganic & Medicinal Chemistry Letters. 2013; 23(13): 3769-3774.
| Physical Appearance | A crystalline solid |
| Storage | Store at -80°C |
| M.Wt | 485.2 |
| Cas No. | 893449-38-2 |
| Formula | C17H11Br2NO2S2 |
| Synonyms | BR-1,P0108,Phosphatase of Regenerating Liver 3 Inhibitor,PTP4A3 Inhibitor |
| Solubility | ≤16mg/ml in DMSO;16mg/ml in dimethyl formamide |
| Chemical Name | 5-[[5-bromo-2-[(2-bromophenyl)methoxy]phenyl]methylene]-2-thioxo-4-thiazolidinone |
| SDF | Download SDF |
| Canonical SMILES | BrC1=CC=C(OCC2=C(Br)C=CC=C2)C(/C=C3C(NC(S\3)=S)=O)=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
