PD 150606
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PD 150606是钙蛋白酶特异性抑制剂,对mu-calpain和m-calpain的Ki值分别为0.21 μM和0.37 μM[1]。
钙蛋白酶是一种酶,在各种生理过程中发挥重要的作用,包括信号转导、细胞骨架重构、基因表达调控、细胞凋亡和细胞周期进程。实验表明,钙蛋白酶参与到多种病理过程,如肌营养不良症、癌症、糖尿病、阿尔茨海默氏病和多发性硬化[2][3]。
PD 150606是一种强效钙蛋白酶抑制剂,并具有与钙蛋白酶抑制剂III相似的活性。当使用感染了ΔPK(导致三维培养中的细胞生长失败)的A2058、A375和HS578T细胞系进行测试时,给予100 μM的PD 150606,通过抑制钙蛋白酶恢复细胞在软琼脂培养基中的三维生长[2]。
注射脂多糖前,先对无病原体的成年C57BL/6小鼠腹腔注射3 mg/kg的PD 150606,以建立具有较低心肌钙蛋白酶活性的败血症小鼠模型。结果表明,PD 150606防止脂多糖诱导的心肌Hsp90/p-Akt蛋白降解,以及抑制心肌caspase-3激活和细胞凋亡[3]。
参考文献:
[1]. Wang, K.K., et al., An alpha-mercaptoacrylic acid derivative is a selective nonpeptide cell-permeable calpain inhibitor and is neuroprotective. Proc Natl Acad Sci U S A, 1996. 93(13): p. 6687-92.
[2]. Colunga, A., et al., Calpain-dependent clearance of the autophagy protein p62/SQSTM1 is a contributor to DeltaPK oncolytic activity in melanoma. Gene Ther, 2014. 21(4): p. 371-8.
[3]. Li, X., et al., The role of the Hsp90/Akt pathway in myocardial calpain-induced caspase-3 activation and apoptosis during sepsis. BMC Cardiovasc Disord, 2013. 13: p. 8.
Physical Appearance | An off-white solid |
Storage | Store at -20°C |
M.Wt | 306.12 |
Cas No. | 179528-45-1 |
Formula | C9H7IO2S |
Solubility | Soluble in DMSO |
Chemical Name | (Z)-3-(4-iodophenyl)-2-sulfanylprop-2-enoic acid |
SDF | Download SDF |
Canonical SMILES | C1=CC(=CC=C1C=C(C(=O)O)S)I |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |