Papain Inhibitor
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Papain Inhibitor是木瓜蛋白酶抑制剂，是具有序列H-Gly-Gly-Tyr-Arg-OH的肽，MW 为451.4。木瓜蛋白酶是一种存在于木瓜和山木瓜中的半胱氨酸蛋白酶。作用机制是通过His-159裂解去质子化的Cys-25肽键。Asparagine-175有助于His-159的咪唑环定向而发生去质子化。Cys-25攻击肽骨干的羰基碳，使肽的氨基末端游离，形成一个共价酰基酶中间体。这个酶通过水分子脱酰基，并释放肽的羧基端部分。众所周知在免疫学中，木瓜蛋白酶可将Fab(抗原结合的)部分和免疫球蛋白(抗体)的Fc部分裂解[1]。木瓜蛋白酶倾向于在(hydrophobic)-(Arg或Lys)间裂解(不是Val)。Hydrophobic是Ala、Val、Leu、Ile、Phe、Trp或Tyr[2]。木瓜蛋白酶可在细胞培养第一步中分离细胞。也可用作各种enzymatic debriding培养基（特别是Accuzyme）的成分。这些都是一些慢性伤口的护理来清除坏死组织。木瓜蛋白酶也可作为一些牙膏或牙齿增白的成分。
Figure1 Formula of Papain Inhibitor peptide
Figure2 Structure of Papain
参考文献：
1. Rawlings ND,Barrett AJ (1994). "Families of cysteine peptidases". Meth. Enzymol. 244: 461–486.
2. Lopes MC,Mascarini RC,da Silva BM,Flório FM,Basting RT (2007). "Effect of a papain-based gel for chemomechanical caries removal on dentin shear bond strength". J Dent Child (Chic) 74 (2): 93–7.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 451.48 |
| Cas No. | 70195-20-9 |
| Formula | C19H29N7O6 |
| Synonyms | H2N-Gly-Gly-Tyr-Arg-OH |
| Solubility | insoluble in EtOH; ≥45.1 mg/mL in DMSO; ≥90.4 mg/mL in H2O |
| Chemical Name | (2S)-2-[[(2R)-2-[[2-[(2-aminoacetyl)amino]acetyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-5-(diaminomethylideneamino)pentanoic acid |
| SDF | Download SDF |
| Canonical SMILES | C1=CC(=CC=C1CC(C(=O)NC(CCCN=C(N)N)C(=O)O)NC(=O)CNC(=O)CN)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
