L-Histidinol (hydrochloride)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
L-Histidinol is an intermediate in the biosynthesis of the amino acid L-histidine.
L-Histidine biosynthesis is a metabolic pathway present in bacteria, archaea, lower eukaryotes, as well as plants. L-histidine biosynthesis has been studied mainly in Escherichia coli and Salmonella typhimurium, revealing fundamental regulatory processes in bacteria.
In vitro: Preliminary experiments were done to investigate the cytotoxic effect of L-histidinol on cultured EAC cells. Results showed that L-histidinol up to 4 mM had no cytotoxic effects on EAC cells. Doxorubicin alone showed concentration-dependent cytotoxic effects. L-Histidinol combination with doxorubicin led to significant potentiation of doxorubicin cytotoxicity to EAC cells compared to doxorubicin alone. The concentration that caused 50% growth inhibition in EAC cells after 24 h incubation was about 0.2 μg/ml, whereas it was 0.1 μg/ml when L-histidinol was added to culture medium [1].
In vivo: L-Histidinol at 250 mg/kg for five consecutive doses before doxorubicin single injection could enhance the antitumour activity of doxorubicin in EAC-bearing mice as demonstrated by a significant increase in average life span and cure rate of mice. In normal mice, L-histidinol, in the same dose regimen, could not alter the acute cardiotoxicity and lethality of doxorubicin [1].
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Al-Shabanah OA, Badary OA, Al-Gharably NM, Al-Sawaf HA. Effects of L-histidinol on the antitumour activity and acute cardiotoxicity of doxorubicin in mice. Pharmacol Res. 1998 Sep;38(3):225-30.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 214.1 |
Cas No. | 1596-64-1 |
Formula | C6H11N3O·2HCl |
Synonyms | (S)-Histidinol |
Solubility | ≤5mg/ml in DMSO |
Chemical Name | (βS)-amino-1H-imidazole-5-propanol, dihydrochloride |
SDF | Download SDF |
Canonical SMILES | OC[C@@H](N)CC1=CNC=N1.Cl.Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |