HZ-1157
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
HZ-1157是针对HCV NS3 / 4A蛋白酶的有效丙型肝炎病毒(HCV)抑制剂,IC50值为1.0 μmol/ L [1].
HCV的NS3蛋白酶是抗HCV药物开发的主要靶标,因为它切割病毒多聚蛋白并释放NS3,NS4A,NS4B,NS5A和NS5B,使其能够在病毒RNA复制中正常发挥功能,而且NS3蛋白酶能使很多参与先天免疫的宿主蛋白失活,以创造有利的细胞环境促进HCV复制.当与其辅因子NS4A复合时,NS3蛋白酶最有活性[1].
体外:建立稳定转染HCV NS3 / 4A蛋白酶报告基因的Huh7.5.1细胞系,来研究HZ-1157(0.001〜1 μmol/ L)的抗HCV机制.结果显示,HZ-1157对HCV NS3 / 4A蛋白酶具有抑制作用,IC50值为1.0 μmol/ L.进一步检测HZ-1157对感染性HCV病毒(J399EM)的作用,J399EM在体外可以感染Huh7.5.1细胞并进行复制.结果显示,在Huh7.5.1细胞中HZ-1157有效抑制J399EM病毒的复制(IC50值为0.82 μmol/ L)[1].
Reference:
[1]. Yu Y, Jing J, Tong X, et al. Discovering novel anti-HCV compounds with inhibitory activities toward HCV NS3/4A protease[J]. Acta Pharmacologica Sinica, 2014, 35(8): 1074.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 232.28 |
| Cas No. | 1009734-33-1 |
| Formula | C12H16N4O |
| Solubility | insoluble in H2O; ≥11.6 mg/mL in DMSO; ≥8.82 mg/mL in EtOH |
| Chemical Name | 5-(tert-butoxy)quinazoline-2,4-diamine |
| SDF | Download SDF |
| Canonical SMILES | NC1=NC(N)=NC2=CC=CC(OC(C)(C)C)=C21 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
