Enoxolone
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
甘草次酸（enoxolone）是甘草的主要组成部分，同时具有抗病毒和抗真菌活性。它能够羧化 DNA的复制并抑制微生物毒素和酶的生成[1,2]。在大鼠中，18β-甘草次酸有效抑制肝和肾匀浆中11β羟基类固醇脱氢酶 (11β-HSD) 的活性，IC50值分别为0.09 μM和0.36 μM[3]。
在糖皮质激素结合盐皮质激素受体（MR）之前，MR对醛固酮的选择性可通过酶诱导，因此导致竞争性的糖皮质激素失活。11β-HSD-1、11β-HSD-2和11β-HSD-3是11β-HSD的三种酶。11β-HSD-1具有双向活性，Km值在微摩尔范围内，是NADP+依赖性的一种酶。11β-HSD-2仅表现出氧化酶的活性，Km值在纳摩尔范围，是NAD +依赖的一种酶。它与MR在肾脏中共定位[4]。
JEG-3细胞系衍生自人绒毛膜癌。在该细胞系中，11β-HSD-3的酶活性被甘草次酸抑制[4]。
在大鼠中，200 mg/kg的甘草次酸口服给药3小时后，显著抑制了肾脏和肝脏中11β-HSD活性。此外，甘草次酸可以稍微增加循环的皮质酮水平。数据表明，11β-HSD同功酶的抑制活性有一定的差异，11-、24-和30-甘草次酸可能发挥重要作用[3]。
参考文献：
[1]. Salari MH, Sohrabi N, Kadkhoda Z, et al. Antibacterial Effects of Enoxolone on Periodontopathogenic and Capnophilic Bacteria Isolated from Specimens of Periodontitis Patients. Iranian Biomedical Journal, 2003, 7(1):39-42.
[2]. Bahmani M, Rafieian-Kopaei M, Jeloudari M, et al. A review of the health effects and uses of drugs of plant licorice (Glycyrrhiza glabra L.) in Iran. Asian Pacific Journal of Tropical Disease, 2015, 5: 127-29.
[3]. Shimoyama Y, Hirabayashi K, Matsumoto H, et al. Effects of glycyrrhetinic acid derivatives on hepatic and renal 11beta-hydroxysteroid dehydrogenase activities in rats. J Pharm Pharmacol, 2003, 55(6):811-7.
[4]. Gomez-Sanchez EP, Cox D, Foecking M, et al. 11 beta-hydroxysteroid dehydrogenases of the choriocarcinoma cell line JEG-3 and their inhibition by glycyrrhetinic acid and other natural substances. Steroids, 1996, 61(3):110-5.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 470.68 |
| Cas No. | 471-53-4 |
| Formula | C30H46O4 |
| Solubility | insoluble in H2O; ≥19.3 mg/mL in DMSO; ≥26.5 mg/mL in EtOH |
| Chemical Name | (2S,4aS,6aS,6bR,8aR,10S,12aS,12bR,14bR)-10-hydroxy-2,4a,6a,6b,9,9,12a-heptamethyl-13-oxo-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,12b,13,14b-icosahydropicene-2-carboxylic acid |
| SDF | Download SDF |
| Canonical SMILES | CC([C@]1([H])CC[C@]23C)([C@](O)([H])CC[C@@]1([C@@]2([H])C(C=C4[C@]5([H])C[C@](CC[C@](CC[C@]43C)5C)(C(O)=O)C)=O)C)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
