DMPO
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
DMPO is a kind of water soluble nitric oxide spin trap, which allows the measurement of oxygen-centered free radicals in biological systems at room temperature using electron spin resonance (ESR). [1]
It has a high reaction rate constant for superoxide and hydroxyl radicals, and distinguishes simultaneously among a variety of important biologically generated free radicals. [1][2]
In vitro, It was demonstrated in red blood cells and in hamster V79 cells that the DMPO spin adducts of ·O2− and ·OH are metabolized very rapidly so that even if formed, they may not be detected in many experiments with cells.[1]
In vivo, when DMPO perfused the heart at 100 μm/L, it attenuated the development of reperfusion arrhythmias during the first 10 min of reperfusion and improved the functional recovery of the heart during reperfusion. Without treatment, 55% of hearts showed irreversible ventricular fibrillation, and this was completely prevented by DMPO, due to its function of traping·O2− and ·OH radicals.[2]
References:
[1] Samuni A1, Samuni A, Swartz HM. The cellular-induced decay of DMPO spin adducts of ·O2− and ·OH. Free Radic Biol Med. 1989; 6(2):179-83.
[2] Tosaki A1, Blasig IE, Pali T, et al. Heart protection and radical trapping by DMPO during reperfusion in isolated working rat hearts. Free Radic Biol Med. 1990; 8 (4):363-72.
Physical Appearance | A colorless oil |
Storage | Store at -20°C |
M.Wt | 113.16 |
Cas No. | 3317-61-1 |
Formula | C6H11NO |
Solubility | ≥11.3 mg/mL in DMSO; ≥55.9 mg/mL in H2O; ≥65.8 mg/mL in EtOH |
Chemical Name | 2,2-dimethyl-3,4-dihydro-2H-pyrrole 1-oxide |
SDF | Download SDF |
Canonical SMILES | [O-][N+]1=CCCC1(C)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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