Alfacalcidol
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Alfacalcidol是一种维生素D的类似物和有效的骨吸收抑制剂[1].
Alfacalcidol可结合钙相关靶器官如肠\骨\肾和甲状旁腺中的VDR(维生素D受体),参与骨骼与矿物质的动态平衡.此外,在OVX后的高骨周转阶段,Alfacalcidol在不引起血钙过多的药理学剂量下可抑制破骨细胞骨吸收.除此之外,Alfacalcidol在OVX模型中给定的血浆和尿液钙水平下可提高骨量和骨强度.
参考文献：
[1] Shiraishi A1, Takeda S, Masaki T, Higuchi Y, Uchiyama Y, Kubodera N, Sato K, Ikeda K, Nakamura T, Matsumoto T, Ogata E. Alfacalcidol inhibits bone resorption and stimulates formation in an ovariectomized rat model of osteoporosis: distinct actions from estrogen. J Bone Miner Res. 2000 Apr;15(4):770-9.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 400.65 |
| Cas No. | 41294-56-8 |
| Formula | C27H44O2 |
| Solubility | insoluble in H2O; ≥20.05 mg/mL in DMSO; ≥21.6 mg/mL in EtOH |
| Chemical Name | (1R,3S,5E)-5-[(2Z)-2-[(1R,3aS,7aR)-7a-methyl-1-[(2R)-6-methylheptan-2-yl]-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexane-1,3-diol |
| SDF | Download SDF |
| Canonical SMILES | CC(C)CCCC(C)C1CCC2C1(CCCC2=CC=C3CC(CC(C3=C)O)O)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 动物实验[1]: | |
|
动物模型 |
卵巢切除大鼠骨质疏松模型 |
|
溶解方法 |
在DMSO中的溶解度>20.1mg/mL。为了获得更高的浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
|
剂量 |
0.05, 0.1和0.2 μg/kg；口服给药；每周5次，给药12周 |
|
应用 |
在卵巢切除大鼠骨质疏松模型中，Alfacalcidol以剂量依赖的方式抑制破骨细胞骨吸收，维持甚至刺激骨形成。Alfacalcidol比17b-雌二醇更有效地增加骨矿物质密度和改善机械强度。 |
|
注意事项 |
请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同，这是由实验系统的误差引起的，属于正常现象。 |
|
References: [1] Shiraishi A1, Takeda S, Masaki T, Higuchi Y, Uchiyama Y, Kubodera N, Sato K, Ikeda K, Nakamura T, Matsumoto T, Ogata E. Alfacalcidol inhibits bone resorption and stimulates formation in an ovariectomized rat model of osteoporosis: distinct actions from estrogen. J Bone Miner Res. 2000 Apr;15(4):770-9. | |
质量控制和MSDS
- 批次:
化学结构
