7-Ethoxyresorufin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
细胞色素P450,家族1,亚家族A,多肽1是由人类CYP1A1基因所编码的一种蛋白.该蛋白属于细胞色素P450超家族.CYP1A1参与I相外源性物质与药物代谢.CYP1A1受喹诺酮类和大环内酯类药物抑制,并受芳香烃诱导.7-Ethoxyresorufin是CYP1A的特异性底物.
体外:7-Ethoxyresorufin(2 ~ 10 microM)是细胞色素P450的底物,结合酶的血红素部分,呈浓度依赖性地降低对乙酰胆碱的应答,但对其它激动剂无效,表明尽管7-Ethoxyresorufin不影响L-精氨酸转化为L-瓜氨酸,但对一氧化氮的合成有效[1].
体内:测定并比较肝脏中CYP1A(7-Ethoxyresorufin)的活性.在小鼠中,LPS在低剂量下(而非在增加促炎细胞因子以及NO血清水平的浓度)抑制CYP2A5活性,并抑制CYP1A活性.促炎细胞因子的抑制能持续下调CYP2A5,而不影响LPS诱导的CYP1A抑制[2].
临床试验:从吸烟者与非吸烟者的人肝穿刺活检样本中测定7-乙氧基试卤灵O-脱乙基酶(ERDE)的CYP450酶活性.在吸烟者的肝脏中,ERDE活性显著上升.血浆Cotinine浓度与ERDE活性之间没有相关性[3].
参考文献：
[1] Oyekan AO, McGiff JC, Rosencrantz-Weiss P, Quilley J. Relaxant responses of rabbit aorta: influence of cytochrome P450 inhibitors. J Pharmacol Exp Ther. 1994 Jan;268(1):262-9.
[2] De-Oliveira AC, Poa KS, Totino PR, Paumgartten FJ. Modulation of cytochrome P450 2A5 activity by lipopolysaccharide: low-dose effects and non-monotonic dose-response relationship. PLoS One. 2015 Jan 30;10(1):e0117842.
[3] Pelkonen O, Pasanen M, Kuha H, Gachalyi B, Kairaluoma M, Sotaniemi EA, Park SS, Friedman FK, Gelboin HV. The effect of cigarette smoking on 7-ethoxyresorufin O-deethylase and other monooxygenase activities in human liver: analyses with monoclonal antibodies. Br J Clin Pharmacol. 1986 Aug;22(2):125-34.
| Physical Appearance | Solid |
| Storage | Store at -20°C |
| M.Wt | 241.24 |
| Cas No. | 5725-91-7 |
| Formula | C14H11NO3 |
| Solubility | Soluble in DMSO |
| Chemical Name | 7-ethoxy-3H-phenoxazin-3-one |
| SDF | Download SDF |
| Canonical SMILES | CCOC1=CC2=C(N=C3C=CC(C=C3O2)=O)C=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
