2'3'-cGAMP (sodium salt)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Cell Counting Kit-8 (CCK-8)
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SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
2’,3’-cGAMP (sodium salt) is a second messenger generated by the enzyme cGAMP synthase (cGAS) in the cytoplasm of mammalian cells, in response to the presence of double-stranded DNA. As a second messenger, 2’,3’-cGAMP sodium salt activates innate immune responses via binding of STING (stimulator of IFN genes) and initiates the TBK1-IRF3-dependent production of IFN-β.
It is used to identify small compounds that bind to STING. It is also applicable in the research of type I interferon response to cytosolic DNA. The binding affinity of 2’,3’-cGAMP to STING is 3.79 nM (Kd value), which is stronger than other cyclic di-nucleotides, e.g. cyclic di-GMP, 3’2’-cGAMP, 3’3’-cGAMP and 2’2’-cGAMP (Kds = 1.21 μM, 1.61 μM, 1.04 μM and 287 nM, respectively).
References:
Zhang X. et al., 2013. Cyclic GMP-AMP containing mixed phosphdiester linkages is an endogenous high-affinity ligand for STING. Mol Cell.51(2):226-35.
Samira Khiar et al., 2017. Identification of a small molecule that primes the type I interferon response to cytosolic DNA. Sci Rep, 7 (1), 2561
Zhong B. et al., 2008. The adaptor protein MITA links virus-sensing receptors to IRF3 transcription factor activation. Immunity. 29(4):538-50.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 718.37 |
Cas No. | 1441190-66-4 |
Formula | C20H22N10Na2O13P2 |
Synonyms | Guanosine-Adenosine 2',3'-cyclic monophosphate,2'-3'-Cyclic GMP-AMP |
Solubility | insoluble in EtOH; insoluble in DMSO; ≥7.56 mg/mL in H2O |
Chemical Name | adenylyl-(3'→5')-2'-guanylic acid, cyclic nucleotide, disodium salt |
SDF | Download SDF |
Canonical SMILES | O=P1([O-])O[C@]([C@@H](O)[C@H](N2C=NC3=C2N=CN=C3N)O4)([H])[C@@]4([H])COP(O[C@@]5([H])[C@H](N6C=NC7=C6NC(N)=NC7=O)O[C@]([C@H]5O)([H])CO1)([O-])=O.[Na+].[Na+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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