Thiolutin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
| Physical Appearance | A crystalline solid |
| Storage | Desiccate at -20°C |
| M.Wt | 228.28 |
| Cas No. | 87-11-6 |
| Formula | C8H8N2O2S2 |
| Solubility | Limited solubility, soluble in DMSO |
| Chemical Name | N-(4-methyl-5-oxodithiolo[4,3-b]pyrrol-6-yl)acetamide |
| SDF | Download SDF |
| Canonical SMILES | CC(=O)NC1=C2C(=CSS2)N(C1=O)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
Human umbilical vein endothelial cells (HUVECs), as well as breast carcinoma (MCF-7), prostate carcinoma (PC3), ovarian carcinoma (CaOV3), and small cell lung carcinoma (OH-1) cells |
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Reaction Conditions |
0.05 ~ 500 μM thiolutin for 72 h incubation |
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Applications |
Thiolutin potently inhibited HUVEC proliferation at 0.1 μM. The potent antiproliferative activity of thiolutin was selective for endothelial cells in that several other tumor cell lines showed weaker inhibition of proliferation by thiolutin (i.e., at 10 μM), and a small cell lung carcinoma cell line was completely resistant. Thus, thiolutin appeared to be a selective inhibitor of endothelial cell proliferation. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Dai S, Jia Y, Wu SL, et al. Comprehensive characterization of heat shock protein 27 phosphorylation in human endothelial cells stimulated by the microbial dithiole thiolutin. Journal of Proteome Research, 2008, 7(10): 4384-4395. |
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化学结构
