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Catalog No. A3008

Y-27632 dihydrochloride

ROCK1抑制剂

Y-27632 dihydrochloride
规格价格货期数量
1mL(10 mM in DMSO)
￥630.00
现货
1mg
￥245.00
现货
2mg
￥345.00
现货
5mg
￥560.00
现货
10mg
￥720.00
现货
25mg
￥1400.00
现货
50mg
￥2599.00
现货
100mg
￥3126.00
现货
200mg
￥4599.00
现货
500mg
￥7199.00
现货
CAS号：129830-38-2纯度：100.00%
仅用于科研，不用于诊疗。未经明确授权不得转售。
A
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特色产品

Annexin V-FITC/PI Apoptosis Kit
Annexin V-FITC/PI Apoptosis Kit
用于双染检测细胞凋亡和坏死
- 在10-20分钟内完成染色
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
Phos binding reagent (Phosbind) acrylamide
Phos binding reagent (Phosbind) acrylamide
分离和检测磷酸化/非磷酸化蛋白
- 新型的磷酸盐结合标签和功能分子
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
Cell Counting Kit-8 (CCK-8)
Cell Counting Kit-8 (CCK-8)
用于细胞增殖和细胞毒性试验
- 比MTT，MTS或WST-1更敏感
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
Cy5 TSA Fluorescence System Kit
Cy5 TSA Fluorescence System Kit
Cy5荧光标记的酪胺信号放大系统
- 检测ICC/IHC/ISH中的低丰度靶点
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP)
EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP)
用于观察mRNA运送，定位，翻译
- 具有Cap1结构的荧光素酶mRNA
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
HotStart™ 2X Green qPCR Master Mix
HotStart™ 2X Green qPCR Master Mix
用于荧光定量目标DNA或cDNA
- 通过热启动机制优化扩增特异性
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit Plus
HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit Plus
高效的Cy5修饰RNA转录试剂盒
- 适合原位/Northernblot杂交实验
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量

产品描述

Y-27632 dihydrochloride是一种Rho相关蛋白激酶p160ROCK的小分子抑制剂，IC50值为140 nM。Y-27632靶向结合ROCK-1和ROCK-2的催化位点，从而抑制它们的激酶活性。因此，Y-27632可以作用于Rho介导的应力纤维的形成，抑制G1-S期细胞周期进程和胞质分裂。

参考文献：
1.  Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. T Ishizaki, M Uehata, I Tamechika, J Keel Molecular Pharmacology 20002. A ROCK inhibitor permits survival of dissociated human embryonic stem cells K Watanabe, M Ueno, D Kamiya, A Nishiyama. Nature Biotechnology. 2007

产品性质

物理外观A solid
CAS号129830-38-2
分子式C14H21N3O·2HCl
分子量320.26
化学名称4-[(1R)-1-aminoethyl]-N-pyridin-4-ylcyclohexane-1-carboxamide
溶解度≥111.2 mg/mL in DMSO; ≥17.57 mg/mL in EtOH; ≥52.9 mg/mL in H2O
SMILESO=C([C@@H]1CC[C@]([C@H](N)C)([H])CC1)NC2=CC=NC=C2.[H]Cl.[H]Cl
存储条件4°C干燥
运输条件蓝冰

产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)

IC50和靶点

生物活性描述Y-27632是一种选择性的ROCK1 (p160ROCK)抑制剂；Ki值为140 nM。比对其他激酶包括PKC、cAMP依赖性蛋白激酶、MLCK和PAK的作用强200多倍。
靶点ROCK1ROCK2
生物活性数据140 nM (Ki)300 nM (Ki)

生物相关数据

质量控制

请选择批次查看:
纯度 = 100.00%
COA (Certificate Of Analysis)HPLCNMR (Nuclear Magnetic Resonance)MSDS (Material Safety Data Sheet)

操作说明

小分子化合物通用使用指南Datasheet

APExBIO 顾客使用本产品发表的 79 篇科研文献

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5. Jun Zhao, Xiaoying Zhang, et al. "Generation of two induced pluripotent stem cell (iPSC) lines (HZSMHCI001-A and HZSMHCI004-A) from a mother–child dyad with major depressive disorder and …." Stem Cell Research Volume 91, March 2026, 103920. PMID: 41643482

6. Bingbing He, Shijian Lv, et al. "Mitigating xenogeneic barriers to chimerism through Cas13-induced host attenuation." Dev Cell. 2026 Jan 29:S1534-5807(26)00001-8. PMID: 41616766

7. Xiaoxin Guo, Gang Du, et al. "Aging-related peroxisomal dysregulation disrupts intestinal stem cell differentiation through alterations of very long-chain fatty acid oxidation." PLoS Biol. 2025 Dec 19;23(12):e3003552. PMID: 41417787

8. Michael S. Sturdivant, Andrew S. Truong, et al. "APOBEC3 promotes squamous differentiation via IL-1A/AP-1 signaling." Nat Commun. 2025 Dec 14;17(1):334. PMID: 41390483

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13. Zehong Zhang, Qianyi Wan, et al. "Lipoic acid functions in Paneth cells to prevent human intestinal stem cell aging." Nat Commun. 2025 Jul 1;16(1):6016. PMID: 40593722

14. Bénédicte Allam-Ndoul, Elena Cristina Pulido-Mateos, et al. "Lactiplantibacillus Plantarum strengthens the intestinal barrier: involvement of the endocannabinoidome." Am J Physiol Gastrointest Liver Physiol. 2025 Aug 1;329(2):G245-G260. PMID: 40522902

15. Hong Wang, Jingyang Qiu, et al. "A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)." Bio Protoc. 2025 May 5;15(9):e5304. PMID: 40364989

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17. Tingyue Luo, Weisheng Chen, et al. "Preclinical models of immune checkpoint inhibitors-related interstitial pneumonia for anti-PD1 tumor immunotherapy." Immunobiology. 2025 Mar;230(2):152884. PMID: 39985972

18. Heidi J. Nick,Sarah E. Christeson, et al. "VX-770, Cact-A1, and Increased Intracellular cAMP Have Distinct Acute Impacts upon CFTR Activity." Int J Mol Sci. 2025, 26(2), 471. PMID: 39859187

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21. Ruize Shi, Mengyi Jin, et al. "Retinoic acid promotes conjunctival epithelium differentiation and goblet cell regeneration: evidence from novel 3D conjunctival organoids and whole-mount PAS staining." Ocul Surf.2025 Jul:37:301-313

22. Lucien Hinderling Remo Hadorn Moritz Kwasny et al. "Teach your microscope how to print: Low-cost and rapid-iteration microfabrication for biology." bioRxiv. February 21, 2025

23. Marlene F. Pereira, Veronica Finazzi, et al. "YY1 mutations disrupt corticogenesis through a cell type specific rewiring of cell-autonomous and non-cell-autonomous transcriptional programs." bioRxiv. 2024 Feb 17:2024.02.16.580337. PMID: 39987231

24. Emily Ballard, Masahiro Sakurai, et al. "Incompatibility in cell adhesion constitutes a barrier to interspecies chimerism." Cell Stem Cell. 2024 Aug 18:S1934-5909(24)00286-8. PMID: 39181131

25. Xiaoxin Guo, Juanyu Zhou, et al. "Very long-chain fatty acids control peroxisome dynamics via a feedback loop in intestinal stem cells during gut regeneration." Dev Cell. 2024 Jul 16:S1534-5807(24)00398-8. PMID: 39047737

26. Xuelian Li, Hongting Du, et al. "FOXL2 regulates RhoA expression to change actin cytoskeleton rearrangement in granulosa cells of chicken pre-ovulatory follicles." Biol Reprod. 2024 Jun 4:ioae082. PMID: 38832713

27. Jessica E. Young, et al. "Differential effects of SORL1 deficiency on the endo-lysosomal network in human neurons and microglia." Philos Trans R Soc Lond B Biol Sci. 2024 Apr 8;379(1899):20220389. PMID: 38368935

28. Heidi J. Nick, Sarah E. Christeson, et al. "The Functional Impact of VX-770 on the Cystic Fibrosis Transmembrane Conductance Regulator Is Enduring and Increases the Constitutive Activity of This Channel in Primary Airway Epithelia Generated from Healthy Donors." Biomolecules 2024, 14(11), 1378

29. Jiahe Li, Shaobo Yang, et al. "In situ neutralization of the genotoxicity of pathogenic gut bacteria with the polyketide synthase island." Research Square. 04 Nov, 2024

30. Viala, Sophie. "Regulation of progenitor cells in epithelial morphogenesis and homeostasis." McGill University

31. Taylor Hinnant, Wenxiu Ning, et al. "Compartment specific responses to contractility in the small intestinal epithelium." bioRxiv. 2023 Aug 7:2023.08.07.552224. PMID: 38517900

32. Jose M de Hoyos-Vega, Xi Yu, et al. "Modeling Gut Neuro-Epithelial Connections in a Novel Microfluidic Device." Microsyst Nanoeng. 2023 Nov 14:9:144. PMID: 38025883

33. Yinju Liu, Jinlong Tan, et al. "A Strainer-Based Platform for the Collection and Immunolabeling of Porcine Epidemic Diarrhea Virus-Infected Porcine Intestinal Organoid." Int J Mol Sci. 2023 Oct 27;24(21):15671. PMID: 37958655

34. Rashmi Chandra, Arpine Sokratian, et al. "Gut mucosal cells transfer α-synuclein to the vagus nerve." bioRxiv. 2023 Aug 14;2023.08.14.553305. PMID: 37645945

35. Niamh McNamee, Mariadelva Catalano, et al. "An extensive study of potential inhibitors of extracellular vesicles release in triple-negative breast cancer." BMC Cancer. 2023 Jul 13;23(1):654. PMID: 37442985

36. Peiyan Ni, Chuqing Zhou, et al. "YBX1‐Mediated DNA Methylation‐DependentSHANK3Expression in PBMCs and Developing Cortical Interneurons in Schizophrenia." Adv Sci (Weinh). 2023 Jul;10(20):e2300455. PMID: 37211699

37. Peiyan Ni, Chuqing Zhou, et al. "Generation and characterization of human-derived iPSC lines from two cousins with schizophrenia and bipolar disorder and their unaffected cousin." Stem Cell Res. 2022 Aug;63:102832. PMID: 35700633

38. Ciaran A Shaughnessy, Pamela L Zeitlin, et al. "Net benefit of ivacaftor during prolonged tezacaftor/elexacaftor exposure in vitro." J Cyst Fibros. 2022 Mar 2;S1569-1993(22)00043-1. PMID: 35248469

39. Peiyan Ni, Huiyao Wang, et al. "Generation and characterization of human-derived iPSC lines from one pair of dizygotic twins discordant for schizophrenia." Stem Cell Res. 2022 Apr;60:102710. PMID: 35182860

40. XiangRong Luo, JianTao She, et al. "Establishment and characterization of organoids from a patient with adenomyoepithelioma of the breast." Bioengineered. 2021 Dec;12(2):11578-11585. PMID: 34874791

41. Changchun Shao, Xue Yang, et al. "The stemness of hepatocytes is maintained by high levels of lipopolysaccharide via YAP1 activation." Stem Cell Res Ther. 2021 Jun 10;12(1):342. PMID: 34112239

42. Chien-Liang Liu, Shih-Ping Cheng, et al. "Quinolinate Phosphoribosyltransferase Promotes Invasiveness of Breast Cancer Through Myosin Light Chain Phosphorylation." Front Endocrinol (Lausanne). 2021 Feb 4;11:621944. PMID: 33613454

43. Tanmoy Mondal, Gururaj N Shivange, et al. "Unexpected PD‐L1 immune evasion mechanism in TNBC, ovarian, and other solid tumors by DR5 agonist antibodies." EMBO Mol Med. 2021 Mar 5;13(3):e12716. PMID: 33587338

44. Leqian Yu, Yulei Wei, et al. "Derivation of Intermediate Pluripotent Stem Cells Amenable to Primordial Germ Cell Specification." Cell Stem Cell. 2021 Mar 4;28(3):550-567.e12. PMID: 33271070

45. Priscilla Cheung, Jordi Xiol, et al. "Regenerative Reprogramming of the Intestinal Stem Cell State via Hippo Signaling Suppresses Metastatic Colorectal Cancer." Cell Stem Cell. 2020 Oct 1;27(4):590-604.e9. PMID: 32730753

46. Nathan D. Jackson, Jamie L. Everman, et al. "Single-Cell and Population Transcriptomics Reveal Pan-epithelial Remodeling in Type 2-High Asthma." Cell Reports;Volume 32, Issue 1, 7 July 2020, 107872. PMID: 32640237

47. Zhao Y, Ge X, et al. "Inhibition of ROCK signaling pathway accelerates enteric neural crest cell-based therapy after transplantation in a rat hypoganglionic model." Neurogastroenterol Motil. 2020;e13895. PMID: 32515097

48. Miller AJ, Yu Q, et al. "In Vitro and In Vivo Development of the Human Airway at Single-Cell Resolution." Dev Cell. 2020;S1534-5807(20)30065-4. PMID: 32109386

49. Andrew S. Riching, Etienne Danis, et al. "Suppression of canonical TGF-β signaling enables GATA4 to interact with H3K27me3 demethylase JMJD3 to promote cardiomyogenesis." bioRxiv. 2020 February 12

50. Allison Knupp, Swati Mishra, et al. "Depletion of the AD risk gene SORL1 selectively impairs neuronal endosomal traffic independent of amyloidogenic APP processing." bioRxiv. 2020 January 06

51. Selvaraj S, Mondragon-Gonzalez R, et al. "Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes." Elife. 2019 Nov 11;8. pii: e47970. PMID: 31710288

52. Tonnessen-Murray CA, Frey WD, et al. "Chemotherapy-induced senescent cancer cells engulf other cells to enhance their survival." J Cell Biol. 2019 Sep 17. pii: jcb.201904051. PMID: 31530580

53. Sagi I, De Pinho JC, et al. "Distinct Imprinting Signatures and Biased Differentiation of Human Androgenetic and Parthenogenetic Embryonic Stem Cells." Cell Stem Cell. 2019 Sep 5;25(3):419-432.e9. PMID: 31491396

54. Cheng CW, Biton M, et al. "Ketone Body Signaling Mediates Intestinal Stem Cell Homeostasis and Adaptation to Diet." Cell. 2019 Aug 22;178(5):1115-1131.e15. PMID: 31442404

55. Alfajaro MM, Kim JY, et al. "Dual Recognition of Sialic Acid and αGal Epitopes by the VP8* Domains of the Bovine Rotavirus G6P[5] WC3 and of Its Mono-reassortant G4P[5] RotaTeq Vaccine Strains." J Virol. 2019 Jun 26. pii: JVI.00941-19. PMID: 31243129

56. Ni P, Noh H, et al. "Large-Scale Generation and Characterization of Homogeneous Populations of Migratory Cortical Interneurons from Human Pluripotent Stem Cells." Mol Ther Methods Clin Dev. 2019 Apr 8;13:414-430. PMID: 31061832

57. Speer JE, Gunasekara DB, et al. "Molecular transport through primary human small intestinal monolayers by culture on a collagen scaffold with a gradient of chemical cross-linking." J Biol Eng. 2019 Apr 27;13:36. PMID: 31061676

58. Ni P, Noh H, et al. "iPSC-derived homogeneous populations of developing schizophrenia cortical interneurons have compromised mitochondrial function." Mol Psychiatry. 2019 Apr 24. PMID: 31019265

59. Dakhama A, Al Mubarak R, et al. "Tollip Inhibits ST2 Signaling in Airway Epithelial Cells Exposed to Type 2 Cytokines and Rhinovirus." J Innate Immun. 2019 Mar 29:1-13. PMID: 30928973

60. Jennifer E. Speer, Yuli Wang, et al. "Evaluation of human primary intestinal monolayers for drug metabolizing capabilities." Journal of Biological Engineering volume 13, Article number: 82 (2019)

61. Rachit Neupane. "BMI1 is a context-dependent tumor suppressor that is a barrier to dedifferentiation in non-small cell lung adenocarcinoma." Massachusetts Institute of Technology. 2019

62. Samuel S. Hinman, Yuli Wang, et al. "Photopatterned Membranes and Chemical Gradients Enable Scalable Phenotypic Organization of Primary Human Colon Epithelial Models." chemrxiv.org. 2019

63. Matthew DiSalvo, Nicole M. Smiddy, et al. "Automated sensing and splitting of stem cell colonies on microraft arrays." APL Bioengineering. 29 August 2019

64. Grun D, Adhikary G, et al. "NRP-1 interacts with GIPC1 and SYX to activate p38 MAPK signaling and cancer stem cell survival." Mol Carcinog. 2018 Nov 19. PMID: 30456845

65. Wang Y, Li Y, et al. "The cerebral cavernous malformation disease causing gene KRIT1 participates in intestinal epithelial barrier maintenance and regulation." FASEB J. 2018 Sep 25:fj201800343R. PMID: 30252535

66. Everman JL, Rios C, et al. "Utilization of Air-Liquid Interface Cultures as an In Vitro Model to Assess Primary Airway Epithelial Cell Responses to the Type 2 Cytokine Interleukin-13." Methods Mol Biol. 2018;1799:419-432. PMID: 29956168

67. Mihaylova MM, Cheng CW, et al. "Fasting Activates Fatty Acid Oxidation to Enhance Intestinal Stem Cell Function during Homeostasis and Aging." Cell Stem Cell. 2018 May 3;22(5):769-778. PMID: 29727683

68. Lin L, Li M, et al. "FPPS mediates TGF-β1-induced non-small cell lung cancer cell invasion and the EMT process via the RhoA/Rock1 pathway." Biochem Biophys Res Commun. 2018 Feb 5;496(2):536-541. PMID: 29337059

69. Roper J, Tammela T, et al. "Colonoscopy-based colorectal cancer modeling in mice with CRISPR-Cas9 genome editing and organoid transplantation." Nat Protoc. 2018 Feb;13(2):217-234. PMID: 29300388

70. Alyssa J. Miller, Quanhui Yu, et al. "Basal stem cell fate specification is mediated by SMAD signaling in the developing human lung." bioRxiv. 2018 November 4

71. MXinwei Feng, Junfeng Lu, et al. "Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells." Front. Cell. Infect. Microbiol., 19 June 2018

72. Wang Y, Kim R, et al. "Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer." Cell Mol Gastroenterol Hepatol. 2017 Nov 3;5(2):113-130. PMID: 29693040

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78. Reynolds SD, Rios C, et al. "Airway Progenitor Clone Formation is Enhanced by Y-27632-dependent Changes in the Transcriptome." Am J Respir Cell Mol Biol. 2016 May 4. PMID: 27144410

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摩尔浓度计算器

质量
= 浓度
x 体积
x 分子量
*在配置溶液时，请务必参考产品标签上、MSDS / COA（可在Apexbio的产品页面获得）批次特异的分子量使用本工具。

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