Spadin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Spadin 是一个强效的TREK-1 channel 抑制剂（IC50=71nM）. 这是一个分泌的多肽，它来自由成熟的NTSR3/Sortilin 生成的前导肽。[1]
TREK1, 又名KCNK2, 是一个双孔蛋白结构域的离子通道，在被动的细胞钾离子跨膜交换中起着作用。 它和局部缺血，感觉缺失和电生成作用有关。它在抑郁症的病理学中和抗抑郁药物的反应中也起着重要作用。[1]
在TREK-1 转染的COS-7细胞中，100nM Spadin 能够抑制63% 的由花生酸刺激产生的TREK-1电流。在野生型小鼠脑切片的CA3海马神经元上, Spadin 也可以阻碍TREK-1 channel的活性。[1]
在预测抗抑郁反应的行为测试中，spadin 给药的小鼠显示出对抑郁的抵抗力。连续给小鼠用药四天，不但表现出很强的抗抑郁效果，并且也助长了海马CREB蛋白的磷酸化和神经系统发育。[2]
参考文献：
1. Borsotto M, Veyssiere J, Moha Ou Maati H et al. Targeting two-pore domain K(+) channels TREK-1 and TASK-3 for the treatment of depression: a new therapeutic concept. Br J Pharmacol. 2015 Feb;172(3):771-84.
2. Mazella J, Pétrault O, Lucas G, et al. Spadin, a sortilin-derived peptide, targeting rodent TREK-1 channels: a new concept in the antidepressant drug design. PLoS Biol. 2010 Apr 13;8(4):e1000355.
| Storage | Store at -20°C |
| M.Wt | 2012.32 |
| Cas No. | 1270083-24-3 |
| Formula | C96H142N26O22 |
| Solubility | Soluble to 2 mg/ml in H2O |
| Chemical Name | (1Z,3S,4Z,7Z,9S,10Z,12S,13Z,15S,16Z,19Z,21S,22Z,24S)-15-((1H-indol-3-yl)methyl)-1-((S)-1-((6S,7Z,9S,10Z,12S,13Z)-9-((1H-indol-3-yl)methyl)-1-amino-6-((Z)-(((S)-1-((S)-2-((Z)-(((S)-1-((S)-2-((Z)-((S)-2-amino-1-hydroxy-3-(4-hydroxyphenyl)propylidene)amino)p |
| SDF | Download SDF |
| Canonical SMILES | CC[C@]([C@@](/N=C(O)/[C@]1([H])CCCN1C(C/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@]2([H])CCCN2C([C@](/N=C(O)/[C@]3([H])CCCN3C([C@](/N=C(O)/[C@](N)([H])CC4=CC=C(O)C=C4)([H])C)=O)([H])CC(C)C)=O)([H])CCCNC(N)=N)([H])CC5=CNC6=CC=CC=C56)([H])CO)=O)([H]) |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
