PKA inhibitor fragment (6-22) amide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ki: 1.6 nM
PKA inhibitor fragment (6-22) amide是一种PKA抑制剂。
在细胞生物学中,蛋白激酶A(PKA)是一种酶家族,其活性依赖于环AMP(cAMP)的细胞水平。PKA也被认为是一种cAMP依赖性蛋白激酶。PKA在细胞中具有多种功能,如糖、糖原和脂质代谢的调节。
体外:与(Ala)Kemptide相比,PKA inhibitor fragment (6-22) amide在抑制效力上具有显著差异,这种效力差异可能是由于几个非精氨酸残基的关键作用。强效抑制cAMP依赖性蛋白激酶的PKA inhibitor fragment (6-22)的最小长度类似物是17残基PKI-(6-22)-amide。PKA inhibitor fragment (6-22)与多肽/蛋白的活性结合部位竞争性地相互作用,具有低纳摩尔Ki值。为了抑制cAMP依赖性蛋白激酶,PKA inhibitor fragment (6-22)需要假底物位点(残基14-22)和额外NH2末端的残基6-13。PKA inhibitor fragment (6-22)与活性位点的结合区特别是COOH末端假底物基本域相互作用,以清除模拟蛋白和肽底物。毫无疑问,PKA inhibitor fragment (6-22)的整体亲和力取决于与cAMP依赖性蛋白激酶的活性位点相互作用的氢键和其它键的数量[1,2]。
体内:N/A
临床试验:N/A
参考文献:
[1] Glasset al(1989) Primary structural determinants essential for potent inhibition of cAMP-dependent protein kinase by inhibitory peptides corresponding to the active portion of the heat-stable inhibitor protein. J.Biol.Chem.2648802. PMID:2722799.
[2] Glasset al(1989) Protein kinase inhibitor-(6-22)-amide peptide analogs with standard and nonstandard amino acid substitutions for phenylalanine 10. J.Biol.Chem.26414579. PMID:2760075.
Physical Appearance | A solid |
Storage | Desiccate at -20°C |
M.Wt | 1868.08 |
Cas No. | 121932-06-7 |
Formula | C80H130N28O24 |
Synonyms | Thr-Tyr-Ala-Asp-Phe-Ile-Ala-Ser-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile |
Solubility | ≥186.8 mg/mL in DMSO; insoluble in EtOH; ≥44.9 mg/mL in H2O |
SDF | Download SDF |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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