Catalog No. A8192

Staurosporine

Name: Staurosporine, 星形孢菌素, 62996-74-1
Brand: APExBIO
SKU: A8192
Price: 1583 CNY
Availability: InStock

（别名：星形孢菌素）

蛋白激酶抑制剂

规格	价格	货期
1mL(10 mM in DMSO)	￥1583.00	现货
1mg	￥420.00	现货
5mg	￥1503.00	现货
10mg	￥2420.00	现货

CAS号：62996-74-1纯度：99.67%

仅用于科研，不用于诊疗。未经明确授权不得转售。

021-55669583sales@apexbio.cnAPExBIO

特色产品

Annexin V-FITC/PI Apoptosis Kit

用于双染检测细胞凋亡和坏死

- 在10-20分钟内完成染色
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞

Phos binding reagent (Phosbind) acrylamide

分离和检测磷酸化/非磷酸化蛋白

- 新型的磷酸盐结合标签和功能分子
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白

Cell Counting Kit-8 (CCK-8)

用于细胞增殖和细胞毒性试验

- 比MTT，MTS或WST-1更敏感
- 对细胞无毒性
- 步骤更简单，无需有机溶剂

Cy5 TSA Fluorescence System Kit

Cy5荧光标记的酪胺信号放大系统

- 检测ICC/IHC/ISH中的低丰度靶点
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率

EZ Cap™ Cy5 Firefly Luciferase mRNA (5-moUTP)

用于观察mRNA运送，定位，翻译

- 具有Cap1结构的荧光素酶mRNA
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰

HotStart™ 2X Green qPCR Master Mix

用于荧光定量目标DNA或cDNA

- 通过热启动机制优化扩增特异性
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单

HyperScribe™ T7 High Yield Cy5 RNA Labeling Kit Plus

高效的Cy5修饰RNA转录试剂盒

- 适合原位/Northernblot杂交实验
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量

产品描述产品性质产品应用生物相关数据质量控制操作说明产品引用文献摩尔浓度计算器

产品描述

Staurosporine（星孢菌素）是一种由链霉菌Streptomyces staurospores产生的生物碱，最初是作为抗真菌剂使用的。Staurosporine是一种广谱性的蛋白激酶抑制剂，可以抑制蛋白激酶C（PKC）、cAMP依赖性蛋白激酶（PKA）、磷酸激酶、核糖体蛋白S6激酶、表皮生长因子受体（EGF-R）激酶和Ca2+/钙调蛋白依赖性蛋白激酶II（钙/钙调PKII）。Staurosporine对PKC的抑制作用是最强的（IC50 = 2.7 nM）。Staurosporine对一些哺乳动物的肿瘤细胞系表现出很强的细胞毒性，诱导细胞凋亡，抑制裂殖酵母在细胞分裂后的伸长。

参考文献：
Takashi Toda, Mizuki Shimanuki, and Mitsuhiro Yanagida. Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases. Genes Dev. 1991 5: 60-73
Michelle M. Hill, Mirjana Andelkovic, Derek P. Brazil, Stefano Ferrari, Doriano Fabbro, and Brian A. Hemmings. Insulin-stimulated protein kinase B phosphorylation on Ser-473 is independent of its activity and occurs through a Staurosporine-insensitive kinase. J. Biol. Chem 2001, 276: 25643-25646.
Flavio Meggio, Arianna Donella Deana, Maria Ruzzene, Anna M. Brunati, Luca Cesaro, Barbara Guerra, Thomas Meyer, Helmut Mett, Doriano Fabbro, Pascal Furet, Grazyna Dobrowolska, and Lorenzo A. Pinna. Different susceptibility of protein kinases to staurosporine inhibition kinetic studies and molecular bases for the resistance of protein kinase CK2. Eur. J. Biochem. 234, 317-322 (1995)

产品性质

物理外观	A solid
CAS号	62996-74-1
分子式	C₂₈H₂₆N₄O₃
分子量	466.53
化学名称	(5S,6R,7R,9R)-6-methoxy-5-methyl-7-(methylamino)-6,7,8,9,15,16-hexahydro-17-oxa-4b,9a,15-triaza-5,9-methanodibenzo[b,h]cyclonona[jkl]cyclopenta[e]-as-indacen-14(5H)-one
溶解度	insoluble in H2O; insoluble in EtOH; ≥11.66 mg/mL in DMSO
SMILES	C[C@@]1([C@@H]([C@@H](C2)NC)OC)O[C@H]2[n]2c3c4[n]1c(cccc1)c1c4c(CNC1=O)c1c3c1c2cccc1
存储条件	-20°C
运输条件	蓝冰

产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)

IC50和靶点

生物活性描述	Staurosporine是一种有效的PKC抑制剂；在非细胞试验中作用于PKCα、PKCγ 和PKCη；IC50分别为2 nM、5 nM 和 4 nM。
靶点	PKCα	PKCγ	PKCη	PKCδ	PKCε	PKCζ
生物活性数据	2 nM	5 nM	4 nM	20 nM	73 nM	1086 nM

生物相关数据

质量控制

请选择批次查看:

纯度 = 99.67%

COA (Certificate Of Analysis)HPLC NMR (Nuclear Magnetic Resonance)MSDS (Material Safety Data Sheet)

操作说明

小分子化合物通用使用指南 Datasheet

APExBIO 顾客使用本产品发表的 22 篇科研文献

1. Kévin Leguay, Omaima Naffati, et al. "TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cells." Life Sci Alliance. 2026 Mar 17;9(6):e202503601. PMID: 41844278

2. Weixiang Liu, Jieming Ping, et al. "Lipid asymmetry disruption by XKR8 orchestrates neutrophil extracellular trap formation and inhibits fungal infection." Nat Immunol. 2026 Mar 4. PMID: 41781710

3. Natalia Gonzalez-Martinez, Ruben M. F. Tomás, et al. "Cryopreservation and post-thaw differentiation of monocytes enabled by macromolecular cryoprotectants which restrict intracellular ice formation." RSC Appl Polym. 2025 Jun 9;3(4):990-1001. PMID: 40503111

4. Genyu Chen, Jian Zhang, et al. "AXL - mediate GEF - H1 phosphorylation was involved in microglia synapse phagocytosis in 5xFAD mice." Exp Neurol. 2025 Oct:392:115327. PMID: 40466990

5. Daniel C Stewart, Becky K Brisson, et al. "Prognostic and therapeutic implications of tumor-restrictive type III collagen in the breast cancer microenvironment." NPJ Breast Cancer. 2024 Oct 2;10(1):86. PMID: 39358397

6. Zongbo Wei, Caili Hao, et al. "Prevention of age-related truncation of γ-glutamylcysteine ligase catalytic subunit (GCLC) delays cataract formation." Sci Adv. 2024 Apr 26;10(17):eadl1088. PMID: 38669339

7. Arwen Conod, Marianna Silvano, et al. "On the origin of metastases: Induction of pro-metastatic states after impending cell death via ER stress, reprogramming, and a cytokine storm." Cell Rep. 2022 Mar 8;38(10):110490. PMID: 35263600

8. Ramon Edwin Caballero, Simon Xin Min Dong, et al. "Role of RIPK1 in SMAC mimetics-induced apoptosis in primary human HIV-infected macrophages." Sci Rep. 2021 Nov 25;11(1):22901. PMID: 34824340

9. Zintis Inde, Jason Rodencal, et al. "Quantification of drug-induced fractional killing using high-throughput microscopy." STAR Protoc. 2021 Jan 22;2(1):100300. PMID: 33532743

10. Chu xue, Si-Xue Liu, et al. "Corydalis Saxicola Bunting Total Alkaloids Inhibits Paclitaxel-Induced Peripheral Neuropathy By Regulating PKCε-TRPV1 and p38 MAPK-TRPV1 Signaling Pathways." Research Square. doi.org/10.21203/rs.3.rs-332970/v1

11. Zintis Inde, Giovanni C. Forcina, et al. "Kinetic Heterogeneity of Cancer Cell Fractional Killing." Cell Rep. 2020 Jul 7;32(1):107845. PMID: 32640215

12. Waer CN, Kaur P, et al. "Rosmarinic Acid/ Blue Light Combination Treatment Inhibits Head and Neck Squamous Cell Carcinoma In Vitro." Anticancer Res. 2020;40(2):751–758. PMID: 32014917

13. Zintis Inde, Giovanni C. Forcina, et al. "Large-Scale Analysis of Cell Death Phenotypic Heterogeneity." bioRxiv 2020.02.28.970079

14. Clark R, Usselmann L, et al. "A flexible high content imaging assay for profiling macrophage efferocytosis." J Immunol Methods. 2019 Jul 29:112636. PMID: 31369739

15. Chung HK, Zou X, et al. "A compact synthetic pathway rewires cancer signaling to therapeutic effector release. Science." 2019 May 3;364(6439) PMID: 31048459

16. Mikhail Chesnokov, Imran Khan, et al. "The MEK1/2 pathway as a therapeutic target in high-grade serous ovarian carcinoma." bioRxiv. 2019 September 16

17. Ashok Kumar, Ramon Edwin Caballero, et al. "Inhibitor of apoptosis, IAP, genes play a critical role in the survival of HIV-infected macrophages." BioRxiv. 2019 February 06

18. Wang Y, Li Y, et al. "The cerebral cavernous malformation disease causing gene KRIT1 participates in intestinal epithelial barrier maintenance and regulation." FASEB J. 2018 Sep 25:fj201800343R. PMID: 30252535

19. Nissen SK, Pedersen JG, et al. "Multiple Homozygous Variants in the STING-Encoding TMEM173 Gene in HIV Long-Term Nonprogressors." J Immunol. 2018 May 15;200(10):3372-3382. PMID: 29632140

20. Jarrett Smith,Geraldine Seydoux, et al. "Liquid-like P granules require ATP hydrolysis to avoid solidification." bioRxiv,Jan. 10, 2018

21. Melanie Villanueva, B.Sc. "Glyoxalase 1 Attenuates the Effects of Chronic Hyperglycemia on Explant-Derived Cardiac Stem Cells." University of Ottawa.Melanie Villanueva, B.Sc

22. Bozza, William P., et al. "The Use of a Stably Expressed FRET Biosensor for Determining the Potency of Cancer Drugs." PloS one 9.9 (2014): e107010. PMID: 25188024

摩尔浓度计算器

质量

= 浓度

x 体积

x 分子量

*在配置溶液时，请务必参考产品标签上、MSDS / COA（可在Apexbio的产品页面获得）批次特异的分子量使用本工具。