OAC3
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
OAC3是八聚体结合转录因子4的一种激活剂。
Octamer结合转录因子4(Oct4)是胚胎干细胞(ESC)多能性的一种关键调节剂,并且Oct4对重编程过程是至关重要的。
体外:OAC3能够激活Oct4和Nanog报告基因至与OAC1类似的程度,OAC1是对Oct4和Nanog启动子驱动的荧光素酶报告基因表现出最大激活作用的OAC类似物。OAC1和OAC3都可以显著增强4F诱导的重编程效率。此外,OAC1和OAC3增强重编程效率四倍,高达2.75%,并且与四种重编程因子(Oct4、Sox2、Klf4和c-Myc )联合用药时还加快iPSC集落的出现3到4d [1]。
体内:为了在动物体内测试4F + OAC2(另一种OAC3类似物)诱导的iPSC的体内多能性,将4F + OAC2-iPSC移植到免疫缺陷的裸鼠中。结果表明,移植后4至6周,4F + OAC2-iPSC可以有效地产生包含三个胚层的衍生物的典型畸胎瘤,例如外胚层的表皮、中胚层的血液以及内胚层的肠上皮[1]。
临床试验:到目前为止,OAC3仍处于临床前研究阶段。
参考文献:
[1] Li W,Tian E,Chen ZX,Sun G,Ye P,Yang S,Lu D,Xie J,Ho TV,Tsark WM,Wang C,Horne DA,Riggs AD,Yip ML,Shi Y. Identification of Oct4-activating compounds that enhance reprogramming efficiency. Proc Natl Acad Sci U S A.2012 Dec 18;109(51):20853-8.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 254.26 |
Cas No. | 182564-41-6 |
Formula | C15H11FN2O |
Solubility | Soluble in DMSO |
Chemical Name | 4-fluoro-N-(1H-indol-5-yl)benzamide |
SDF | Download SDF |
Canonical SMILES | O=C(C1=CC=C(C=C1)F)NC2=CC=C3NC=CC3=C2 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |