O4I1
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
O4I1是Oct3/4的强效诱导剂，促进Oct3/4的表达和稳定。O4I1可以增强多种人类体细胞Oct3/4的转录活性。OCT3/4也被称为POU5F1和Oct4，是一种转录因子，对于胚胎干细胞的自我更新和多能性具有重要作用。OCT3/4在ES细胞、外胚层、前胚胎植入和生殖细胞中有特异性表达[1]。
当从经10 μM和20 μM O4I1处理72小时的HEK293细胞中分离RNA并得到合成的cDNA，发现Oct3/4的mRNA水平分别增加大约2.5和4倍。在成人原代成纤维细胞（HF）和人新生儿包皮成纤维（HFF）细胞系中，经10 μM的O4I1培养HF细胞72小时以及HFF细胞7天后，Oct3/4的基因和蛋白表达水平增高。O4I1的衍生化合物2以时间依赖性的方式激活Oct3/4，显著地提升Oct3/4的诱导效率[1]。
参考文献：
Cheng X, Dimou E, Alborzinia H, et al. Identification of 2-[4-[(4-Methoxyphenyl) methoxy]-phenyl] acetonitrile and Derivatives as Potent Oct3/4 Inducers[J]. Journal of medicinal chemistry, 2015, 58(12): 4976-4983.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 253.3 |
| Cas No. | 175135-47-4 |
| Formula | C16H15NO2 |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥45 mg/mL in DMSO |
| Chemical Name | 2-(4-((4-methoxybenzyl)oxy)phenyl)acetonitrile |
| SDF | Download SDF |
| Canonical SMILES | COC1=CC=C(COC2=CC=C(CC#N)C=C2)C=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
