NB-598
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
| Physical Appearance | A brown oil |
| Storage | Store at -20°C |
| M.Wt | 449.67 |
| Cas No. | 131060-14-5 |
| Formula | C27H31NOS2 |
| Synonyms | NB 598;NB598 |
| Solubility | insoluble in H2O; ≥49.3 mg/mL in EtOH with gentle warming; ≥54 mg/mL in DMSO with gentle warming |
| Chemical Name | (E)-N-(3-([3,3'-bithiophen]-5-ylmethoxy)benzyl)-N-ethyl-6,6-dimethylhept-2-en-4-yn-1-amine |
| SDF | Download SDF |
| Canonical SMILES | CCN(CC1=CC(OCC2=CC(C3=CSC=C3)=CS2)=CC=C1)C/C=C/C#CC(C)(C)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
MIN6 cells, as well as mouse and human pancreatic islets |
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Reaction Conditions |
10 μM NB-598 for 48 h incubation |
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Applications |
Cholesterol reduction of 36 ~ 52% was observed in MIN6 cells, mouse and human pancreatic islets after a 48-h incubation with 10 μM NB-598. A similar reduction in cholesterol was observed in the subcellular compartments of MIN6 cells. In addition, inhibition of cholesterol biosynthesis by NB-598 impaired insulin secretion and voltage-gated calcium channel function in pancreatic beta-cells. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Xia F, Xie L, Mihic A, et al. Inhibition of cholesterol biosynthesis impairs insulin secretion and voltage-gated calcium channel function in pancreatic beta-cells. Endocrinology, 2008, 149(10): 5136-5145. |
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质量控制和MSDS
- 批次:
化学结构
