N3-kethoxal
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 5mg | ￥3181.00 | 现货 | |
| 25mg | ￥9090.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
N3-kethoxal is a small, membrane-permeable probe, which selectively binds to un-paired guanine residues within a cellular environment and then provides a bio-orthogonal handle for click chemistry reactions. Depending on how the azide group is utilized, N3-kethoxal is amenable to various nucleic acid probing applications. The N3-kethoxal platform can be utilized to probe RNA secondary and tertiary structure, map single stranded DNA regions in the genome, explore RNA-RNA interactions, and identify protein-RNA interactions. Compared with current nucleic acid probes, N3-kethoxal exhibits low toxicity, as well as high specificity, resolution, and reproducibility, providing a novel click chemistry platform for in vitro and in vivo analyses of nucleic acid structure and proximity interactions.
References:
1. Huang J, Zhao R, Mo J, et al. N3-kethoxal-based bioorthogonal intracellular RNA labeling. ChemBioChem, 2021, 22(9): 1559-1562.
2. He C. Novel click chemistry platform for in vivo and in vitro analysis of nucleic acid structure and proximity interactions. Polsky Center for Entrepreneurship and Innovation, 2019, reference ID: 19-T-037.
3. Weng X, Gong J, Chen Y, et al. Keth-seq for transcriptome-wide RNA structure mapping. Nature Chemical Biology, 2020, 16(5): 489-492.
产品性质
| 物理外观 | Liquid |
| CAS号 | 2382756-48-9 |
| 分子式 | C6H11N3O4 |
| 分子量 | 189.17 |
| 化学名称 | 3-(2-azidoethoxy)-1,1-dihydroxybutan-2-one |
| 溶解度 | ≥94.6 mg/mL in DMSO; ≥24.6 in H2O; ≥30.4 mg/mL in EtOH |
| SMILES | CC(C(C(O)O)=O)OCCN=[N+]=[N-] |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
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