ML-095 (hydrochloride)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ML-095 (hydrochloride) is a specific PLAP inhibitor [1].
Alkaline phosphatases are hydrolase enzymes responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. There are three isozymes of alkaline phosphatases: placental alkaline phosphatase (PLAP), intestinal alkaline phosphatase (IAP), and tissue non-specific alkaline phosphatase (TNAP). Placental alkaline phosphatase (PLAP) is highly expressed in primate placental tissue and the biological function is still unknown. However, PLAP-like enzymes are detected in serum of patients with primary testicular tumors, in particular seminoma and other cancers [1].
ML-095 (CID-25067483) is a biochemical inhibitor of PLAP and will be useful to elucidate the key biological functions and natural substrates of human PLAP. ML095 is a specific PLAP inhibitor and inhibited PLAP, TNAP and IAP with IC50 values of 2.1, >100 and 53 μM, respectively. ML095 exhibited greater than 25-fold selectivity over tissue non-specific alkaline phosphatase (TNAP) and intestinal alkaline phosphatase (IAP) [1].
Reference:
[1]. Lanier M, Cashman J, Sergienko E, et al. Placental Alkaline Phosphatase (PLAP) Luminescent HTS assay - Probe 2.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 282.7 |
Cas No. | 1135318-57-8 |
Formula | C13H14N2O3·HCl |
Synonyms | CID-25067483 |
Solubility | ≤10mg/ml in DMSO |
Chemical Name | 1-(3,4-dihydroxyphenyl)-2-(2-ethyl-1H-imidazol-1-yl)-ethanone, monohydrochloride |
SDF | Download SDF |
Canonical SMILES | OC1=CC=C(C(CN2C=CN=C2CC)=O)C=C1O.Cl |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |