7-Methoxycoumarin-4-acetyl-P-L-G-L-β-(2,4-dinitrophenylamino)A-R amide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
我们保留了Pro-Leu-Gly-Leu的序列，但在N端标记了高荧光的(7-甲基香豆素-4-) 乙酰基（Mea）基团。Dnp淬灭基团被插入到新的N3-Dnp-t-2,3-二氨基丙酸（Dpa）的P2’位点上[1]，因为基质金属蛋白酶偏好芳香族侧链的P2’位点[2,3]。
在非淬灭肽Mca-Pro-Leu中，最大荧光激发波长为328nm，发射波长在393nm附近。在1 M下比较，Mca-Pro-Leu的荧光强度比Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2强130倍。
Mea-Pro-Leu-Gus)-Lrlt-Dpu-AMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2的剪切产物是目前所知最敏感的基质金属蛋白酶连续测定的底物，不仅是因其Kcut/Km值比以往报道的物质高，由于Mea基团的荧光比色氨酸强，也能被Dnp基团有效淬灭，其剪切产物能被快速检测。除胶原蛋白酶外，新的底物在与其偏好的大分子底物实验所需的相似酶浓度下，连续检测也能进行。因此，Mca-Pro- Leu-Gly-Leu-Dpa-Ala-Arg-NH2适用于粗制剂中总金属蛋白酶活性的检测。后续对可被特定酶切割的多肽的研发将能满足这些条件下对特定酶的检测[3]。
参考文献：
1. Schectcr, I, and Beryer. A. (lYG7) Biochem. Biophyt;. Rcs, Commun. 27, 157-161
2. Nctzcl-Arnctt, s.. Mallya. SK., Nugasc. H.. Birkedal-Hansen, H. and Van Wurt. H.E. (1991) Anal. Biochcm. 195. 86-92.
3. Netzel-Arnctt, S., Fields. G., Birkedal-Hunscn. H. and Vai Wart. H.E. (1991) J. Biol. Chem. 266. 6747-6755
4. C.G.Knight, F. Willenbrock and G. Murphy. A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases. FEDS. Volume 296, number 3, 263-266
| Storage | Store at -20°C |
| M.Wt | 1093.15 |
| Cas No. | 140430-53-1 |
| Formula | C49H68N14O15 |
| Solubility | ≥48.7 mg/mL in DMSO,insoluble in EtOH,insoluble in H2O |
| Chemical Name | (2R)-N-((6S,9S,12S,16R,22R)-1,12-diamino-6-carbamoyl-13-(2,4-dinitrophenyl)-1-imino-16-isobutyl-9,24-dimethyl-8,11,15,18,21-pentaoxo-2,7,10,14,17,20-hexaazapentacosan-22-yl)-1-(2-(7-methoxy-2-oxo-2H-chromen-4-yl)acetyl)pyrrolidine-2-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | COC1=CC=C(C(CC(N2CCC[C@@H]2C(N[C@H](CC(C)C)C(NCC(N[C@H](CC(C)C)C(NC([C@H](C(N[C@@H](C(N[C@@H](C(N)=O)CCCNC(N)=N)=O)C)=O)N)C3=CC=C([N+]([O-])=O)C=C3[N+]([O-])=O)=O)=O)=O)=O)=O)=CC(O4)=O)C4=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
