Ionomycin calcium salt
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
钙离子（钙）信号通路在受精中发挥主要作用，精子不仅提供其遗传物质，也使细胞内钙离子浓度升高，这对于完成卵母细胞活化和胚胎发育是必要的。离子霉素是由链霉菌产生的离子载体。工业上，离子霉素通常为游离酸或钙盐。
体外实验：离子霉素是用于研究胞内钙水平的提高和研究跨膜钙转运的工具。它也可以用来刺激胞内产生细胞因子，如干扰素、穿孔素、IL-2和IL-4。这些细胞因子在炎症反应中发挥重要作用[1]。
在体实验：10 μmol/l的离子霉素有效激活卵母细胞的活化和随后的发育效率。二次给予5 μmol/l离子霉素明显改善囊胚发育。在体外或体内小鼠胚胎发育中使用离子霉素无副作用[1]。
临床试验：截至目前，离子霉素仍处于临床前开发阶段。
参考文献：
[1] Heytens E, Soleimani R, Lierman S, De Meester S, Gerris J, Dhont M, Van der Elst J, De Sutter P. ? Effect of ionomycin on oocyte activation and embryo development in mouse. Reprod Biomed Online. 2008 Dec;17(6):764-71.
| Physical Appearance | A crystalline solid |
| Storage | Desiccate at -20°C |
| M.Wt | 747.08 |
| Cas No. | 56092-82-1 |
| Formula | C41H70O9·Ca |
| Solubility | Soluble in DMSO |
| Chemical Name | calcium (4S,6S,8S,10E,12R,14R,16E,18S,19S,20R,21S)-19,21-dihydroxy-22-((2R,2'R,5R,5'S)-5'-((R)-1-hydroxyethyl)-2,5'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate |
| SDF | Download SDF |
| Canonical SMILES | O[C@H](C)[C@@]1(C)O[C@H](CC1)[C@]2(C)O[C@@H](C[C@@H]([C@@H](C)[C@H]([C@H](/C=C/C[C@@H](C)C[C@@H](C)/C([O-])=C\C([C@@H](C)C[C@@H](C)C[C@@H](C)CCC([O-])=O)=O)C)O)O)CC2.[Ca+2] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
Human bladder cancer cell line HT1376 |
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Reaction Conditions |
0 ~ 100 μg/ml ionomycin for 0 ~ 96 h incubation |
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Applications |
The in vitro growth rate of HT1376 cells was suppressed by ionomycin in a dose- and time-dependent manner. After continuous treatment with 10 μg/ml ionomycin, characteristic apoptotic DNA degradation, as well as marked decreases in the ratios of Bcl-2 to Bax mRNA and protein could be observed in HT1376 cells. |
| Animal experiment:[1] | |
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Animal models |
Athymic nude mice bearing HT1376 tumors |
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Dosage form |
50 μg of ionomycin in 100 μl PBS Injected intratumorally three times weekly for 4 weeks |
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Applications |
Intratumoral injection of ionomycin into subcutaneous HT1376 tumors reduced the tumorigenicity in nude mice. Furthermore, these in vivo growth-inhibitory effects of ionomycin were significantly enhanced by pretreatment with cisplatin. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Miyake H, Hara I, Yamanaka K, et al. Calcium ionophore, ionomycin inhibits growth of human bladder cancer cells both in vitro and in vivo with alteration of Bcl-2 and Bax expression levels. The Journal of Urology, 1999, 162(3 Pt 1): 916-921. |
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化学结构
