Hydrocortisone
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Hydrocortisone is a main glucocorticoid secreted by the adrenal cortex.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 362.46 |
Cas No. | 50-23-7 |
Formula | C21H30O5 |
Solubility | insoluble in H2O; insoluble in EtOH; ≥13.3 mg/mL in DMSO |
Chemical Name | (8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-3-one |
SDF | Download SDF |
Canonical SMILES | C[C@@]1([C@](C(CO)=O)(O)CC2)[C@@]2([C@@]([H])([C@]([H])([C@H](C1)O)[C@@]3(C)CC4)CCC3=CC4=O)[H] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验[1]: | |
细胞系 |
人肺微血管内皮细胞 |
溶解方法 |
在DMSO中的溶解度 >13.3 mg/mL。为了获得更高的浓度,可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
反应条件 |
4或6 μM; 16 h |
应用 |
在人肺微血管内皮细胞中,Hydrocortisone(HC)诱导浓度依赖但生物学上轻微的屏障增强和保护作用。在LPS处理前或处理后,HC(4μM)和抗坏血酸(VitC)(1000μM)的组合显著逆转了LPS诱导的屏障功能障碍。 |
动物实验[2]: | |
动物模型 |
6-羟基多巴胺(6-OHDA)诱导的帕金森病(PD)小鼠模型 |
剂量 |
0.4 mg/kg;腹腔注射,连续给药7天 |
应用 |
在帕金森病(PD)小鼠模型中,Hydrocortisone在选定的脑亚区域(纹状体,腹侧中脑和小脑)中引起parkin蛋白表达量近两倍的增加,这是细胞应对氧化应激存活所必需的。Hydrocortisone也增加了CREB的表达。Hydrocortisone预处理显著增强了6-OHDA中毒的多巴胺能神经元的存活。 |
注意事项 |
请测试所有化合物在室内的溶解度,实际溶解度和理论值可能略有不同,这是由实验系统的误差引起的,属于正常现象。 |
References: [1]. Barabutis N1, Khangoora V2, Marik PE2, et al. Hydrocortisone and Ascorbic Acid synergistically prevent and repair LPS- induced pulmonary endothelial barrier dysfunction. Chest. 2017 Jul 21. pii: S0012-3692(17)31276-X. [2]. Ham S1, Lee YI2,3, Jo M1, et al. Hydrocortisone-induced parkin prevents dopaminergic cell death via CREB pathway in Parkinson's disease model. Sci Rep. 2017 Apr 3;7(1):525. |
质量控制和MSDS
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