GTP-Binding Protein Fragment, G alpha
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
序列:Cys-Gly-Ala-Gly-Glu-Ser-Gly-Lys-Ser-Thr-Ile-Val-Lys-Gln-Met-Lys
在针对合成肽的特异性抗血清实验中,即使在使用非水解的GTP类似物激活后,三个不同的GTP结合蛋白α亚基仍然保持与质膜结合。胰蛋白酶在靠近氨基的末端催化分解每个α亚基,,将除了氨基末端2 kDa片段的其余大片段从膜中定量释放。之前的研究表明,α亚基是胞质内的重要蛋白,通过氨基末端与细胞膜内表面连接。
参考文献:
1. Brock Eide, Peter Gierschik, Graeme Milligan, Ian Mullaney, Cecilia Unson, Paul Goldsmith, Allen Spiegel, Biochemical and Biophysical Research Communications, Volume 148, Issue 3, 13 November 1987, Pages 1398–1405
2. J. Falloon, H. Malech, G. Milligan, C. Unson, R. Kahn, P. Goldsmith, A. Spiegel, FEBS Letters, 209 (1986), pp. 352–356
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 1623.89 |
Formula | C66H118N20O23S2 |
Synonyms | H2N-Cys-Gly-Ala-Gly-Glu-Ser-Gly-Lys-Ser-Thr-Ile-Val-Lys-Gln-Met-Lys-OH |
Solubility | insoluble in EtOH; ≥162.3 mg/mL in DMSO; ≥51.5 mg/mL in H2O |
SDF | Download SDF |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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