FLI-06
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
FLI-06是一种新型的Notch信号通路抑制剂,EC50值为2.3 uM[1].
Notch信号通路在许多细胞的命运决定中起重要作用,其异常活性导致癌症和发育障碍[1].
用FLI-06处理HeLa NotchΔE-eGFP细胞,导致NotchΔE-eGFP累积和NICD-eGFP生成减少.将FLI-06洗脱后,表型在1–4 h内完全可逆,这表明FLI-06对细胞不是极性毒性的[1].在 FLI-06 处理的细胞中,Aβ 分泌显著降低而不是APPCTF 积累,这表明FLI-06 在α-分泌酶和β-分泌酶裂解的上游起作用.对HeLa细胞进行免疫荧光分析显示, FLI-06 引起高尔基体的完全中断,这可通过干扰早期分泌途径的膜转运或通过分解微管17引起.FLI-06抑制ER消退,也引起tubule-to-sheet表型,且彼此相关[1].
参考文献：
[1]. Krmer A, Mentrup T, Kleizen B, et al. Small molecules intercept Notch signaling and the early secretory pathway. Nat Chem Biol, 2013, 9(11): 731-738.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 438.52 |
| Cas No. | 313967-18-9 |
| Formula | C25H30N2O5 |
| Solubility | ≥16.1 mg/mL in DMSO; insoluble in H2O; ≥2.35 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | cyclohexyl 2,7,7-trimethyl-4-(4-nitrophenyl)-5-oxo-1,4,6,8-tetrahydroquinoline-3-carboxylate |
| SDF | Download SDF |
| Canonical SMILES | CC1=C(C(C2=C(N1)CC(CC2=O)(C)C)C3=CC=C(C=C3)[N+](=O)[O-])C(=O)OC4CCCCC4 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
