eukaryotic translation initiation factor 3
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
分子式：C47H84N14O14S。
真核起始因子（eIF）是参与真核翻译起始阶段的蛋白质。eIF3结合到核糖体亚基-mRNA复合物，在延伸准备就绪之前，参与阻止大的核糖体亚基与小亚基的结合。在哺乳动物中，eIF3是最大的染色体支架起始因子（scaffolding initiation factor），由13个亚单位组成（a-m），分子量大约为750 kDa，控制40S核糖体亚基在含有5'帽或IRES(内部核糖体进入位点)的mRNA上组装。 eIF3使用来自病毒的eIF4F复合物或IRES定位到靠近40S核糖体亚单位出口处的mRNA链，从而推动转录起始复合物的组装[1]。
位于8q23-q24处的PTK2和EIF3S3基因可能是扩增靶标，和肝癌有关[2]。在晚期前列腺癌中，普遍存在EIF3S3基因的扩增，它可能功能上参与了疾病的进展[3]。最近的研究表明，在前列腺癌中，真核翻译起始因子3亚基3未出现不依赖于c-myc基因的过表达[4]。
参考文献：
1. Umadas Maitra, Jayanta Chaudhuri; Jayanta Chaudhuri, Kausik Si (21). "Function of Eukaryotic Translation Initiation Factor 1A (eIF1A) (Formerly Called eIF-4C) in Initiation of Protein Synthesis". The Journal of BIological Chemistry 272 (12): 7883–7891. doi:10.1074/jbc.272.12.7883. Retrieved 19 February 2012.
2. PTK2 and EIF3S3 genes may be amplification targets at 8q23-q24 and are associated with large hepatocellular carcinomas. Okamoto, H., Yasui, K., Zhao, C., Arii, S., Inazawa, J. Hepatology (2003) [Pubmed]
3. Amplification of EIF3S3 gene is associated with advanced stage in prostate cancer. Saramäki, O., Willi, N., Bratt, O., Gasser, T.C., Koivisto, P., Nupponen, N.N., Bubendorf, L., Visakorpi, T. Am. J. Pathol. (2001) [Pubmed]
4. Mapping and gene expression profile of the minimally overrepresented 8q24 region in prostate cancer. Tsuchiya, N., Kondo, Y., Takahashi, A., Pawar, H., Qian, J., Sato, K., Lieber, M.M., Jenkins, R.B. Am. J. Pathol. (2002) [Pubmed]
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 1101.32 |
| Formula | C47H84N14O14S |
| Synonyms | H2N-Asn-Leu-Gln-Leu-Leu-Met-Asp-Arg-Val-OH |
| Solubility | ≥110.1 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
| SDF | Download SDF |
| Canonical SMILES | NC(CC(N)=O)C(NC(CC(C)C)C(NC(CCC(N)=O)C(NC(CC(C)C)C(NC(CC(C)C)C(NC(CCSC)C(NC(CC(O)=O)C(NC(CCCNC(N)=N)C(NC(C(C)C)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
