E6 Berbamine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
E6 Berbamine is a calmodulin inhibitor.
Calmodulin, the ubiquitous and multifunctional Ca2+-binding protein, can mediate various regulatory effects of Ca2+, such as the contractile state of smooth muscle. In smooth muscle, the main function of calmodulin is to activate crossbridge cycling and the development of force in response to Ca2+ transient through the activation of myosin light-chain kinase and myosin phosphorylation.
In vitro: The results from a previous study showed that the inhibition of MLCK activity could be increased with increasing levels of E6 Berbamine and was completely overcome by the addition of excessive Ca2+. In addition, the stimulatory activity of MLCK induced by Ca2+ was gradually inhibited by the increasing concentrations of E6 Berbamine suggesting that the inhibition of MLCK activity by E6 Berbamine was concentration dependent. In addition, E6 Berbamine could diminish the fluorescence intensity of dansyl-labeled Ca2+. E6 Berbamine showed no effect on the activity of MLCK fragments produced by limited trypsinization, a novel and considerably potent calmodulin antagonist [1].
In vivo: So far, there is no animal in vivo data reported.
Clinical trial: Up to now, E6 Berbamine is still in the preclinical development stage.
Reference:
[1] Z. Y. Hu, Y. S. Gong and W. L. Huang. Interaction of berbamine compound E6 and calmodulin-dependent myosin light chain kinase. Biochemical Pharmacology44(8), 1543-1547 (1992).
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 757.8 |
Cas No. | 114784-59-7 |
Formula | C44H43N3O9 |
Synonyms | Berbamine p-nitrobenzoate,O-(4-Nitrobenzoyl)berbamine |
Solubility | ≤25mg/ml in ethanol;25mg/ml in DMSO |
Chemical Name | 3,4,4aS,5,16aR,17,18,19-octahydro-21,22,26-trimethoxy-4,17-dimethyl-16H-1,24:6,9-dietheno-11,15-metheno-2H-pyrido[2’,3’:17,18][1,11]dioxacycloeicosino[2,3,4-ij]isoquinolin-12-ol-4-nitrobenzoate |
SDF | Download SDF |
Canonical SMILES | COC1=C(C2=C(CCN([C@]2([H])CC3=CC=C(OC(C4=CC=C([N+]([O-])=O)C=C4)=O)C(OC5=CC=C(C[C@@]67[H])C=C5)=C3)C)C=C1OC)OC8=CC6=C(CCN7C)C=C8OC |
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