AS 1269574
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AS 1269574，（2-[2-(4-溴苯基)-6-甲基嘧啶-4-基]氨基乙醇），可在正常小鼠中诱导葡萄糖刺激的胰岛素分泌（GSIS）及提高葡萄糖耐受。AS 1269574是GPR119的激动剂，EC50值为2.5 μM[1][2]。
GPR119在胰腺β细胞中高表达。该受体通过提高胞内cAMP浓度，增强GSIS效果[2]。
以1或10 μM的AS 1269574处理20 min，16.8 mM葡萄糖预处理的小鼠胰腺MIN-6 β细胞的胰岛素分泌与DMSO溶剂组相比显著提高。相比之下，AS 1269574不可影响低浓度葡萄糖（2.8 mM）预处理的MIN-6细胞的胰岛素释放[2]。
在正常小鼠中，以100 mg/kg的AS 1269574单一处理可显著降低2 h后（AUC0-2h）的血糖的曲线下面积（AUC）。AS 1269574相比溶剂对照可显著提高小鼠的血浆胰岛素AUC0-2h。单一口服100 mg/kg的AS 1269574不能显著降低禁食或喂食小鼠的葡萄糖AUC，表明AS 1269574不具有降血糖作用[2]。
参考文献：
[1]. Shigeru Yoshida, Hirotsugu Tanaka, Hiroyuki Oshima, et al. AS1907417, a novel GPR119 agonist, as an insulinotropic and β-cell preservative agent for the treatment of type 2 diabetes. Biochemical and Biophysical Research Communications, 2010, 400: 745-751.
[2]. Shigeru Yoshida, Takahide Ohishi, Tetsuo Matsui, et al. Identification of a novel GPR119 agonist, AS1269574, with in vitro and in vivo glucose-stimulated insulin secretion. Biochemical and Biophysical Research Communications, 2010, 400: 437-441.
| Physical Appearance | A solution in ethanol |
| Storage | Store at -20°C |
| M.Wt | 308.17 |
| Cas No. | 330981-72-1 |
| Formula | C13H14BrN3O |
| Solubility | Soluble in DMSO |
| Chemical Name | 2-((2-(4-bromophenyl)-6-methylpyrimidin-4-yl)amino)ethanol |
| SDF | Download SDF |
| Canonical SMILES | BrC1=CC=C(C=C1)C2=NC(C)=CC(NCCO)=N2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
