Aminoallyl-UTP
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Aminoallyl-UTP用于基于核酸序列的扩增(NASBA),基于基因芯片的杂交检测,Eberwine mRNA扩增和RNA标记。 在NASBA反应和Eberwine mRNA扩增过程中,Aminoallyl-UTP被插入RNA序列,产生的胺官能化的RNA可以通过经典的伯胺/ NHS酯反应来引入荧光基团(通过荧光染料的NHS酯),用于随后基于基因芯片的杂交检测。
参考文献:
[1] Scheler et al. (2009) Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications. BMC Biotechnol. 9:45.
[2] Hoen et al. (2003) Fluorescent labelling of cRNA for microarray applications. Nucleic Acids Res. 31 (5):e20.
Physical Appearance | Solution |
Storage | Store at -20°C or below |
M.Wt | 536.19(Anion) |
Cas No. | 112131-73-4 |
Formula | C12H17N3O15P3 (Anion) |
Synonyms | 5-(3-Aminoallyl)-uridine-5'-triphosphate,aa-utp,aa utp,5-AA-UTP,5-Aminoallyluridine-5'-Triphosphate |
Solubility | Soluble in sterile water |
Chemical Name | ((2R,3S,4R,5R)-5-(5-((E)-3-aminoprop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate |
SDF | Download SDF |
Canonical SMILES | O=C(N1)N([C@@H]2O[C@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)[C@@H](O)[C@H]2O)C=C(/C=C/CN)C1=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |