Z-WEHD-FMK
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
用pan-caspase抑制剂IV和炎性caspases抑制剂Z-WEHD-FMK处理感染的细胞,几乎完全阻断了沙眼衣原体引起的golgin-84的裂解。通过使用Z-WEHD-FMK处理感染的细胞阻断golgin-84的裂解,导致高尔基碎片的缺失和一些感染性细菌2-log的减少。
用Z-WEHD-FMK处理感染细胞,有效地阻止了高尔基体裂解,用DMSO作为对照,然后用荧光神经酰胺标记细胞。共聚焦图像显示,在DMSO处理的细胞中,神经酰胺迅速进入包涵体膜,并且在细菌膜内包涵体中积累。相反,Z-WEHD-FMK处理的细胞只有轻微的荧光,因为大多数脂质在包涵体外的高尔基体样结构中积累[1]。
通常的caspase抑制剂(Z-Asp-CHz-DCB)和capase-5抑制剂(Z-WEHD-FMK)不能诱导ECyd处理的rRNA裂解。Caspase-5(ICErei Ill/TY),是ICE蛋白酶的成员,激活的通路可能与ECyd诱导rRNA裂解有关[2]。
参考文献:
[1]. D. Heuer, A.R. Lipinskiet al.Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction.NATURE, 457, 2009
[2]. Kamada, S., Funahashi, Y. and Tsujimoto, Y. (1997)Cell Death Diff. 4, 473-478.
- 1. Shouwen Chen, Peng Jin, et al. "Dual function of a turbot inflammatory caspase in mediating both canonical and non-canonical inflammasome activation." Dev Comp Immunol. 2021 Aug;121:104078. PMID:33794278
- 2. WeiWei, Xiao-XueLi, et al. "Inhibition of vascular neointima hyperplasia by FGF21 associated with FGFR1/Syk/NLRP3 inflammasome pathway in diabetic mice." Atherosclerosis. Available online 29 August 2019.
- 3. Yang D, Zheng X, et al. "Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish." Nat Commun. 2018 Aug 3;9(1):3052. PMID:30076291
Storage | Store at -20°C |
M.Wt | 763.77 |
Cas No. | 210345-00-9 |
Formula | C37H42FN7O10 |
Synonyms | Z-Trp-Glu(OMe)-His-Asp(OMe)-FMK |
Solubility | insoluble in H2O; ≥26.32 mg/mL in EtOH with ultrasonic; ≥46.33 mg/mL in DMSO |
Chemical Name | methyl (4S)-5-[[(2S)-1-[[(3S)-5-fluoro-1-methoxy-1,4-dioxopentan-3-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-4-[[(2S)-3-(1H-indol-3-yl)-2-(phenylmethoxycarbonylamino)propanoyl]amino]-5-oxopentanoate |
SDF | Download SDF |
Canonical SMILES | COC(=O)CCC(C(=O)NC(CC1=CN=CN1)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CC2=CNC3=CC=CC=C32)NC(=O)OCC4=CC=CC=C4 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Cell experiment:[1] | |
Cell lines |
Chlamydia trachomatis (C. trachomatis)-infected HeLa cells |
Reaction Conditions |
80 μM Z-WEHD-FMK for 9 h incubation |
Applications |
Z-WEHD-FMK (80 μM; 9 h) elicited a near-complete blockage of C. trachomatis-induced cleavage of golgin-84 and increased GM130 expression in cells. Z-WEHD-FMK treatment resulted in a 2-log reduction in numbers of infectious bacteria, which could be reversed by knocking down golgin-84. Thus, Z-WEHD-FMK could effectively prevent Golgi fragmentation, and was used to explore the role of fragmentation of the Golgi compartment in Chlamydia reproduction. |
Note |
The technical data provided above is for reference only. |
References: 1. Heuer D, Rejman Lipinski A, Machuy N, et al. Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction. Nature, 2009, 457(7230): 731-735. |
一种合成肽,不可逆地抑制caspase-5及相关caspase的活性。 | ||||||
靶点 | caspase-5 | |||||
IC50 |
质量控制和MSDS
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