XL335
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
XL335是法尼酯X受体（FXR）的有效的、选择性可口服激动剂，EC50值为4 nM[1]。
在人肝癌Hep3B细胞中，XL335通过作用于FXR，减少IL-6诱导的CRP的mRNA和蛋白表达。在野生型（WT）小鼠中，XL335显着减少LPS诱导的SAP和SAA3 mRNA的表达，而在FXR/ KO小鼠中没有变化[2]。
另外，在肝癌细胞中，XL335阻断棕榈酸（PA）诱导的脂质积聚。在体内，XL335减少小鼠中门静脉内毒素的水平以及果糖诱发的炎症。XL335减弱脂多糖（LPS）诱导小鼠中的炎症，抑制ADRP的表达[3]。
参考文献：
[1] Flatt B1,Martin R,Wang TL,Mahaney P,Murphy B,Gu XH,Foster P,Li J,Pircher P,Petrowski M,Schulman I,Westin S,Wrobel J,Yan G,Bischoff E,Daige C,Mohan R. DiscoveryofXL335(WAY-362450), ahighlypotent,selective, andorallyactiveagonistof thefarnesoidXreceptor (FXR). J Med Chem.2009 Feb 26;52(4):904-7. doi: 10.1021/jm8014124.
[2] Zhang S1,Liu Q,Wang J,Harnish DC. Suppressionofinterleukin-6-inducedC-reactiveproteinexpressionbyFXRagonists. Biochem Biophys Res Commun.2009 Feb 6;379(2):476-9.
[3] Liu X1,Xue R2,Ji L1,Zhang X1,Wu J3,Gu J1,Zhou M4,Chen S5. Activation of farnesoid X receptor (FXR) protects against fructose-inducedliver steatosis via inflammatory inhibition and ADRP reduction. Biochem Biophys Res Commun.2014 Jul 18;450(1):117-23.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 438.48 |
| Cas No. | 629664-81-9 |
| Formula | C25H24F2N2O3 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥20.4 mg/mL in DMSO |
| Chemical Name | propan-2-yl 3-(3,4-difluorobenzoyl)-1,1-dimethyl-2,6-dihydroazepino[4,5-b]indole-5-carboxylate |
| SDF | Download SDF |
| Canonical SMILES | CC(C)OC(=O)C1=CN(CC(C2=C1NC3=CC=CC=C32)(C)C)C(=O)C4=CC(=C(C=C4)F)F |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
