TWS119
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
TWS119是一种糖原合酶激酶-3β(GSK-3β)的抑制剂,IC50值为30 nM[1].
TWS119是从一个吡咯并嘧啶化合物的文库中筛选出来的,在小鼠P19 EC细胞中可选择性诱导神经元分化.TWS119可紧密结合GSK-3β,Kd值为126 nM,抑制能力的IC50值为30 nM.TWS119与GSK-3β的结合可调控复合体的活性,并可触发下游转录事件,从而引起神经诱导.除此之外,TWS119可通过另一机制,而非典型的Wnt信号通路,促进mESCs的神经元分化[1,2].
参考文献：
[1] Ding S, Wu T Y H, Brinker A, et al. Synthetic small molecules that control stem cell fate. Proceedings of the National Academy of Sciences, 2003, 100(13): 7632-7637.
[2] Wu T Y H, Ding S. Applying chemical tools to the discovery of novel regenerative medicine. Drug Discovery Today: Technologies, 2006, 3(3): 255-260.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 318.33 |
| Cas No. | 601514-19-6 |
| Formula | C18H14N4O2 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥15.9 mg/mL in DMSO |
| Chemical Name | 3-[[6-(3-aminophenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy]phenol |
| SDF | Download SDF |
| Canonical SMILES | C1=CC(=CC(=C1)N)C2=CC3=C(N2)N=CN=C3OC4=CC=CC(=C4)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | TWS119是一种GSK-3β抑制剂,IC50值为30 nM. | |||||
| 靶点 | GSK-3β | |||||
| IC50 | 30 nM | |||||
质量控制和MSDS
- 批次:
化学结构
相关生物数据
