Tenofovir Disoproxil Fumarate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Tenofovir disoproxil fumarate是一种核酸逆转录酶抑制剂,可在MT-2细胞和PBMC中抑制人类免疫缺陷病毒(HIV)的复制,半数最大有效浓度EC50值分别为0.007 μmol/L和0.005 μmol/L[1]。
施药后不久,tenofovir disoproxil fumarate迅速通过酯酶水解脱去两个酯基生成tenofovir,一种具有抗病毒活性的核苷酸类似物,可拮抗逆转录病毒包括HIV-1、HIV-2和嗜肝DNA病毒。Tenofovir可被细胞的核苷酸激酶和腺苷酸激酶磷酸化,最终生成其活性代谢产物tenofovir二磷酸,可抑制HIV-1逆转录酶,并能终止DNA链延伸[2]。
参考文献:
[1] Fung HB, Stone EA, Piacenti FJ. Tenofovir disoproxil fumarate: a nucleotide reverse transcriptase inhibitor for the treatment of HIV infection. Clin Ther. 2002 Oct;24(10):1515-48.
[2] Brian P. Kearney, John F. Flaherty, Jaymin Shah. Tenofovir Disoproxil Fumarate. Clinical Pharmacokinetics. August 2004, Volume 43, Issue 9, pp 595-612
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 635.51 |
Cas No. | 202138-50-9 |
Formula | C19H30N5O10P·C4H4O4 |
Solubility | ≥31.78 mg/mL in DMSO; ≥2.615 mg/mL in H2O with gentle warming and ultrasonic; ≥8.48 mg/mL in EtOH with ultrasonic |
Chemical Name | [[(2R)-1-(6-aminopurin-9-yl)propan-2-yl]oxymethyl-(propan-2-yloxycarbonyloxymethoxy)phosphoryl]oxymethyl propan-2-yl carbonate;(E)-but-2-enedioic acid |
SDF | Download SDF |
Canonical SMILES | CC(C)OC(=O)OCOP(=O)(COC(C)CN1C=NC2=C1N=CN=C2N)OCOC(=O)OC(C)C.C(=CC(=O)O)C(=O)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1]: | |
细胞系 |
MT-2细胞和PBMCs |
制备方法 |
在DMSO中的溶解度大于31.8 mg/mL。若配制更高浓度的溶液,一般步骤如下:请将试管置于37 °C加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20 °C可放置数月。 |
反应条件 |
0.007 μM或0.005 μM |
实验结果 |
在MT-2细胞和PBMC中,Tenofovir Disoproxil Fumarate抑制HIV复制,其EC50值分别为0.007 μM和0.005 μM。Tenofovir Disoproxil Fumarate的抗HIV活性比Tenofovir高36 ~ 90倍。 |
References: [1]. Fung HB, Stone EA, Piacenti FJ. Tenofovir disoproxil fumarate: a nucleotide reverse transcriptase inhibitor for the treatment of HIV infection. Clin Ther. 2002 Oct;24(10):1515-48. |
质量控制和MSDS
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