Filipin III
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Filipin III是一种广泛用于胆固醇在膜上定位的荧光探针。与胆固醇结合后， Filipin III的荧光光谱特性发生改变。Ex/Em = 340-380/385-470 nm.
胆固醇既是细胞膜的重要结构成分，又是激素和胆汁酸生物合成的早期中间体。菲律宾菌素（Filipin）是从菲律宾链霉菌（S. filipinensis）培养物中分离得到的四种异构多烯大环内酯类混合物的统称。Filipin III是主要的异构体（53%），也是大多数研究中使用的异构体。作为天然荧光多烯抗生素，Filipin III可与胆固醇结合[1,2]，但不与酯化固醇结合，被广泛用来检测膜中游离的（即未酯化的）胆固醇[3]。最大激发光波长约360 nm，最大发射光波长约480 nm。
References：
[1] Volpon L, Lancelin J. Solution NMR structures of the polyene macrolide antibiotic filipin III. FEBS Lett. 2000;478(1-2):137–140.
[2] Bittman R, Fischkoff SA. Fluorescence studies of the binding of the polyene antibiotics filipin 3, amphotericin B, nystatin, and lagosin to cholesterol. Proc Natl Acad Sci U S A. 1972;69(12):3795–3799.
[3] Maxfield FR, Wüstner D. Analysis of cholesterol trafficking with fluorescent probes. Methods Cell Biol. 2012;108:367–393.
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| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C, protect from light. 该产品在溶液中不稳定，建议避光分装保存，避免反复冻融，尽快使用。 |
| M.Wt | 654.83 |
| Cas No. | 480-49-9 |
| Formula | C35H58O11 |
| Solubility | Soluble in DMSO |
| Chemical Name | (3R,4S,6S,8S,10R,12R,14R,16S,17E,19E,21E,23E,25E,27S,28R)-4,6,8,10,12,14,16,27-octahydroxy-3-((R)-1-hydroxyhexyl)-17,28-dimethyloxacyclooctacosa-17,19,21,23,25-pentaen-2-one |
| SDF | Download SDF |
| Canonical SMILES | O[C@@H]1/C(C)=C/C=C/C=C/C=C/C=C/[C@H](O)[C@@H](C)OC([C@@]([C@H](O)CCCCC)([H])[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C1)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验 [1]: | |
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细胞系 |
含麦角固醇的纤毛膜 |
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溶解方法 |
该化合物可溶于DMSO。若获取更高浓度的溶液，可在37°C下孵育10分钟，随后在超声波浴中摇匀。 |
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反应条件 |
12.7 μM |
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应用 |
Filipin诱导卵磷脂-胆固醇和卵磷脂-麦角固醇囊泡的裂解，但不溶解卵磷脂单独制备的囊泡和卵磷脂与表皮固醇、硫代胆固醇、雄激素-3/3-醇或胆甾醇的混合物形成的囊泡。与Filipin治疗的卵磷脂-麦角甾醇囊泡相比，Filipin治疗的含麦角固醇的睫状肌膜产生的环状物的平均直径几乎相同。 |
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注意事项 |
由于实验环境的不同，实际溶解度可能与理论值略有不同，请测试室内所有化合物的溶解度。 |
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References: [1]. Bittman R, Chen W C, Anderson O R. Interaction of filipin III and amphotericin B with lecithin-sterol vesicles and cellular membranes. Spectral and electron microscope studies[J]. Biochemistry, 1974, 13(7): 1364-1373. |
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化学结构
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