SR3335
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SR 3335是合成的RORα的选择性反向激动剂，Ki值为220 nM[1,2]。
视黄酸受体相关孤儿受体α（RORα）是一种孤儿受体，在代谢的调节中发挥重要作用。
SR3335阻止HepG2肝癌中内源性RORα的表达。SR3335可能抑制2型糖尿病患者肝葡萄糖的生成。SR3335能够抑制葡萄糖-6-磷酸酶（G6Pase）的表达。在ChIP沉淀实验中，ML-176减少G6Pase启动子上SRC2的量[3]。在GAL4-RORR配体结合结构域共转染实验中，SR3335抑制RORα的组成型活性，Ki值为220 nM[1]。
在饮食诱导的肥胖小鼠中，注射丙酮酸后，使用15 mg/kg的SR3335进行治疗，与对照组相比，每个时间点的血浆葡萄糖水平显著降低，表明SR3335可抑制肝糖原异生[1]。
参考文献：
[1]. Naresh Kumar, Douglas J. Kojetin, et al. Identification of SR3335 (ML176): a Synthetic RORα Selective Inverse Agonist. ACS Chem Biol. 2011 March 18; 6(3): 218-222.
[2]. Laura A. Solt, Thomas P. Burris. Action of RORs and their ligands in (patho)physiology. Trends in Endocrinology & Metabolism, 2012, 23(12): 619-627.
[3]. Kumar N, Nuhant P, Solt LA, et al. Identification of a novel selective inverse agonist probe and analogs for the Retinoic acid receptor-related Orphan Receptor Alpha (RORα). Probe Reports from the NIH Molecular Libraries Program [Internet], Bethesda (MD): National Center for Biotechnology Information (US),2010-.2010
.| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 405.34 |
| Cas No. | 293753-05-6 |
| Formula | C13H9F6NO3S2 |
| Synonyms | ML-176;SR 3335;SR-3335;ML176;ML 176 |
| Solubility | insoluble in H2O; ≥87.4 mg/mL in EtOH; ≥88.8 mg/mL in DMSO |
| Chemical Name | N-[4-(1,1,1,3,3,3-hexafluoro-2-hydroxypropan-2-yl)phenyl]thiophene-2-sulfonamide |
| SDF | Download SDF |
| Canonical SMILES | C1=CSC(=C1)S(=O)(=O)NC2=CC=C(C=C2)C(C(F)(F)F)(C(F)(F)F)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 描述 | SR3335是视黄酸受体相关孤儿受体（ROR）的选择性反向激动剂，对RORα的IC50值为480 nM。 | |||||
| 靶点 | RORα | |||||
| IC50 | 480 nM | |||||
质量控制和MSDS
- 批次:
化学结构
