RF 9
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
RF9对NPFF1和NPFF2的Ki值分别为58 nM和75 nM。
RF9是一种神经肽FF受体拮抗剂。
神经肽FF（NPFF）与软体动物心脏兴奋肽FMRF-NH2的C末端序列相似，与软体动物心脏兴奋肽FMRF-NH2的抗体发生交叉反应，最初从牛脑中分离。最近研究表明，NPFF属于包括2种G蛋白偶联受体（NPFF1和NPFF2）以及2种前体（pro-NPFFA和pro-NPFFB）的神经肽家族。体内外研究表明，NPFF参与一系列生物学功能。
体外：RF9是一种有效的NPFF受体选择性拮抗剂。RF9能选择性地结合CHO或COS-1细胞的重组NPFF1或NPFF2受体。在功能研究中，RF9体外拮抗NPFF和NPVF诱导的激动作用[1]。
体内：小鼠体内研究表明，注射RF9（30 nmol）未能引起显著作用，但颅内注射RF9可以完全拮抗NPFF引起的低温症。此外，同时在第三脑室注射RF9（30 nmol）能减少吗啡或痛敏肽/孤啡肽引起的体温过低[2]。
临床试验：N/A。
参考文献：
[1] Fang Q, Wang YQ, He F, Guo J, Guo J, Chen Q, Wang R. Inhibition of neuropeptide FF (NPFF)-induced hypothermia and anti-morphine analgesia by RF9, a new selective NPFF receptors antagonist. Regul Pept. 2008 Apr 10;147(1-3):45-51.
[2] Wang YQ, Guo J, Wang SB, Fang Q, He F, Wang R. Neuropeptide FF receptors antagonist, RF9, attenuates opioid-evoked hypothermia in mice. Peptides. 2008 Jul;29(7):1183-90.
| Physical Appearance | White lyophilised solid |
| Storage | Store at -20°C |
| M.Wt | 482.62 |
| Cas No. | 876310-60-0 |
| Formula | C26H38N6O3 |
| Solubility | Soluble in DMSO |
| Chemical Name | (3S,5S,7S,Z)-N-((S,Z)-5-guanidino-1-hydroxy-1-(((S)-1-hydroxy-1-imino-3-phenylpropan-2-yl)imino)pentan-2-yl)adamantane-1-carbimidic acid |
| SDF | Download SDF |
| Canonical SMILES | N=C(O)[C@](/N=C(O)/[C@](/N=C(O)/C12C[C@@]3([H])C[C@](C1)([H])C[C@](C2)([H])C3)([H])CCCNC(N)=N)([H])CC4=CC=CC=C4 |
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