PX 12
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PX 12是硫氧还蛋白1(Trx-1)的抑制剂[1]。
硫氧还蛋白1(TRX-1)是一个小型氧化还原蛋白,含有一个保守的催化位点,在细胞中发挥重要作用,包括调节反式激活活性和氧化还原敏感性转录因子的DNA结合[1]。
在HT-29人结肠癌细胞和MCF-7人乳腺癌中,PX 12阻止缺氧诱导的HIF-1蛋白的增加。另外,PX 12减少诱导型一氧化氮合酶、HIF-1反式激活活性和VEGF的形成[2]。
在HT-29人结肠癌异种移植免疫缺陷小鼠中,在2小时内,PX 12将平均肿瘤血管透过性降低63%,48小时后恢复到治疗前水平。PX 12处理24小时减少肿瘤源性血管内皮生长因子和肿瘤。另外,在2小时内,Trx-1出现快速下降,可以维持24小时[1]。在MCF-7肿瘤异种移植小鼠中,PX 12治疗减少HIF-1ɑ和VEGF蛋白含量[2]。在癌症患者中,PX-12治疗显著降低血浆中的Trx-1和VEGF的含量[3]。
参考文献:
[1]. Jordan BF, Runquist M, Raghunand N, et al. The thioredoxin-1 inhibitor 1-methylpropyl 2-imidazolyl disulfide (PX-12) decreases vascular permeability in tumor xenografts monitored by dynamic contrast enhanced magnetic resonance imaging. Clin Cancer Res, 2005, 11(2 Pt 1): 529-536.
[2]. Welsh SJ, Williams RR, Birmingham A, et al. The thioredoxin redox inhibitors 1-methylpropyl 2-imidazolyl disulfide and pleurotin inhibit hypoxia-induced factor 1alpha and vascular endothelial growth factor formation. Mol Cancer Ther, 2003, 2(3): 235-243.
[3]. Baker AF, Dragovich T, Tate WR, et al. The antitumor thioredoxin-1 inhibitor PX-12 (1-methylpropyl 2-imidazolyl disulfide) decreases thioredoxin-1 and VEGF levels in cancer patient plasma. J Lab Clin Med, 2006, 147(2): 83-90.
Storage | Store at 4°C |
M.Wt | 188.31 |
Cas No. | 141400-58-0 |
Formula | C7H12N2S2 |
Solubility | insoluble in H2O; ≥8.75 mg/mL in DMSO; ≥99 mg/mL in EtOH |
Chemical Name | 2-(butan-2-yldisulfanyl)-1H-imidazole |
SDF | Download SDF |
Canonical SMILES | CCC(C)SSC1=NC=CN1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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