O-Phospho-D-Serine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 1,260 μM for the metabotropic glutamate receptor mGluR4
O-Phospho-D-Serine is a weak agonist of the metabotropic glutamate receptor mGluR4.
mGluR4 receptors, a member of the G protein-linked family of receptors for glutamate, are negatively coupled to cAMP. Its expression is highest in granule cells of the cerebellum that send parallel fibers that synapse with Purkinje cell dendrites.
In vitro: The affinity of L-serine-O-phosphate was examined in a baby hamster kidney cells expressing subtype mGluR1 or mGluR4 of the mGluR family. Results showed that both L-serine-O-phosphate or O-Phospho-D-Serine at 3 mM did not inhibit PI-hydrolysis as stimulated by 10 μM glutamate in BHK cells expressing mGluR1. However, L-serine-O-phosphate was a potent agonist at the mGluR4 subtype, which was negatively coupled to adenylate cyclase, while O-Phospho-D-Serine was found to be weakly active [1]. In another study, to test whether phosphatidylserine was recognized by amebae, calcium-treated cells were incubated with annexin V prior to adherence to or ingestion by E. histolytica. It was found that the coreceptor could be inhibited by phospho-l-serine and to a lesser extent by O-Phospho-D-Serine but not by phospho-l-threonine, which was consistent with the coreceptor functioning in the adherence and ingestion of erythrocytes via recognition of phosphatidylserine [2].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
References:
[1] Thomsen, C. , and Suzdak, P.D. Serine-O-phosphate has affinity for type IV, but not type I, metabotropic glutamate receptor. NeuroReport 4, 1099-1101 (1993).
[2] Boettner, D. R.,Huston, C.D.,Sullivan, J.A., et al. Entamoeba histolytica and Entamoeba dispar utilize externalized phosphatidylserine for recognition and phagocytosis of erythrocytes. Infection and Immunity 73(6), 3422-3430 (2005).
Storage | Store at -20°C |
M.Wt | 185.1 |
Cas No. | 73913-63-0 |
Formula | C3H8NO6P |
Synonyms | D-O-Phosphoserine,D-Serine-O-Phosphate,D-SOP |
Solubility | insoluble in DMSO; insoluble in EtOH; ≥18.5 mg/mL in H2O with gentle warming and ultrasonic |
Chemical Name | O-phosphono-D-serine |
SDF | Download SDF |
Canonical SMILES | O=C(O)[C@H](N)COP(O)(O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |