Myriocin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Myriocin是一种有效的免疫抑制剂,也是一种特异性的丝氨酸棕榈酰转移酶抑制剂,Ki值为0.28 nM [1][2]。
丝氨酸棕榈酰转移酶催化从头神经鞘脂生物合成的第一步。鞘脂参与调节细胞凋亡、细胞增殖、细胞迁移、衰老和炎症[2]。
Myriocin是一种特异性的丝氨酸棕榈酰转移酶抑制剂。在肺癌A549和NCI-H460细胞中,myriocin (10、20和50 µM)以浓度依赖的方式抑制细胞增值。Myriocin抑制A549和NCI-H460细胞生长的IC50值分别为30 µM和26 µM[4]。
在黑色素瘤小鼠中,myriocin(1 mg/kg,隔天给药,共给药3周)皮下或腹腔给药显著抑制肿瘤形成。Myriocin也减少肿瘤组织中Cdc25C、Cdc2和cyclin B1的表达,增加p53和p21waf1/cip1的表达。Myriocin也减少血清、肝和肿瘤组织中鞘脂的水平[3]。在BALB/c小鼠中,myriocin增加纹状体和海马中多巴胺(DA)的水平,减少皮质中DA的水平[2]。
参考文献:
Miyake Y, Kozutsumi Y, Nakamura S, et al. Serine palmitoyltransferase is the primary target of a sphingosine-like immunosuppressant, ISP-1/myriocin. Biochem Biophys Res Commun. 1995 Jun 15;211(2):396-403.
Osuchowski MF, Johnson VJ, He Q, et al. Myriocin, a serine palmitoyltransferase inhibitor, alters regional brain neurotransmitter levels without concurrent inhibition of the brain sphingolipid biosynthesis in mice. Toxicol Lett. 2004 Feb 28;147(1):87-94.
Lee YS, Choi KM, Lee S, et al. Myriocin, a serine palmitoyltransferase inhibitor, suppresses tumor growth in a murine melanoma model by inhibiting de novo sphingolipid synthesis. Cancer Biol Ther. 2012 Jan 15;13(2):92-100.
Choi KE, Jung YS, Kim DH, et al. Myriocin induces apoptotic lung cancer cell death via activation of DR4 pathway. Arch Pharm Res. 2014 Apr;37(4):501-11.
- 1. Ting Wang, Jingmin Zhang, et al. "Lipidomics Analysis Reveals Protective Effect of Myriocin on Cerebral Ischemia/Reperfusion Model Rats." Research Square. rs-1324750/v1.
- 2. Xue-Ming Zhu, Lin Li, et al. "A VASt-domain protein regulates autophagy, membrane tension, and sterol homeostasis in rice blast fungus." Autophagy. 2020 Nov 11. PMID:33176558
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 401.54 |
Cas No. | 35891-70-4 |
Formula | C21H39NO6 |
Solubility | 2mg/mL in methanol |
Chemical Name | (2S,3R,4R,E)-2-amino-3,4-dihydroxy-2-(hydroxymethyl)-14-oxoicos-6-enoic acid |
SDF | Download SDF |
Canonical SMILES | CCCCCCC(CCCCCC/C([H])=C([H])/C[C@](O)([H])[C@@](O)([H])[C@@](C(O)=O)(N)CO)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |