Mupirocin
异亮氨酰t-RNA合成酶抑制剂
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 1mL(10 mM in DMSO) | ￥425.00 | 现货 | |
| 10mg | ￥459.00 | 现货 | |
| 25mg | ￥827.00 | 现货 | |
| 100mg | ￥2646.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
Mupirocin is an antibiotic that inhibits isoleucyl-tRNA synthetase in bacterial cell walls, weakening the growth of bacteria with an MIC value of 0.05 µg/ml in Staphylococcus aureus.It is mainly used as an antibacterial agent against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA).
MRSA can resist therapeutic treatment via causing chronic infections leading to high rate mortality, by causing a proteic biofilm, extracellular DNA or carbohydrate matrix that allows bacterial survival in unfavourable environments.
Because of the similarity between isoleucine and the antibiotics's epoxy side chain, Mupirocin can prevent the conversion of isoleucine and tARN to isoleucine-charged transfer RNA by binding the active centre of isoleucyl-tRNA syntethase and thus decreasing its cellular levels inside the cells, causing bacterial death.
Mupirocin is a strong acid as a form of a crystalline white powder, freely soluble in dichloromethane and acetone, slightly soluble in water. Its antibiotic activity is pH dependent, at pH between 4 and 9 in aqueous solutions, the activity is maintained; but outside this interval, molecular rearrangements occur with the formation of two isomers, without interconversion. Mupirocin is the most widely used topical antibiotic to control the MRSA outbreaks, for infections of soft tissue or skin and for nasal decolonization.
References:
1. Tucaliuc A, Blaga AC, Galaction AI, Cascaval D. Mupirocin: applications and production. Biotechnol Lett. 2019 May;41(4-5):495-502. doi:10.1007/s10529-019-02670-w. Epub 2019 Mar 29. Review. PubMed PMID: 30927135.
2. Khoshnood S, Heidary M, Asadi A, Soleimani S, Motahar M, Savari M, Saki M, Abdi M. A review on mechanism of action, resistance, synergism, and clinical implications of mupirocin against Staphylococcus aureus. Biomed Pharmacother. 2019 Jan; 109:1809-1818. doi: 10.1016/j.biopha.2018.10.131. Epub 2018 Nov 26.Review. PubMed PMID: 30551435.
3. Poovelikunnel T, Gethin G, Humphreys H. Mupirocin resistance: clinical implications and potential alternatives for the eradication of MRSA. J Antimicrob Chemother. 2015 Oct;70(10):2681-92. doi: 10.1093/jac/dkv169. Epub 2015 Jul 3. Review. PubMed PMID: 26142407.
4. Hughes J, Mellows G. Inhibition of isoleucyl-transfer ribonucleic acid synthetase in Escherichia coli by pseudomonic acid. Biochem J. 1978 Oct 15;176(1):305-18. PubMed PMID: 365175; PubMed Central PMCID: PMC1186229.
产品性质
| 物理外观 | A solid |
| CAS号 | 12650-69-0 |
| 分子式 | C26H44O9 |
| 分子量 | 500.62 |
| 化学名称 | 9-[(E)-4-[(2S,3R,4R,5S)-3,4-dihydroxy-5-[[(2S,3S)-3-[(2S,3S)-3-hydroxybutan-2-yl]oxiran-2-yl]methyl]oxan-2-yl]-3-methylbut-2-enoyl]oxynonanoic acid |
| 溶解度 | ≥100 mg/mL in DMSO; insoluble in H2O; ≥5.69 mg/mL in EtOH |
| SMILES | O[C@@H]1[C@H](CO[C@@H](C/C(C)=C/C(OCCCCCCCCC(O)=O)=O)[C@@H]1O)C[C@@H]2O[C@H]2[C@@H](C)[C@H](C)O |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)
IC50和靶点
| 生物活性描述 | 莫匹罗星（BRL-4910A；Pseudomonic acid）是从荧光假单胞菌中分离出来的一种口服活性抗生素。莫匹罗星显然是通过可逆地抑制异亮氨酰转移核糖核酸；从而抑制细菌蛋白质和核糖核酸的合成来发挥其抗菌活性的。 |
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