ML188
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ML188是一种选择性的非共价SARS CoV 3CLpro抑制剂,分子量适中,具有良好的酶和抗病毒抑制活性(IC50 = 1.5 μM)。在细胞培养中进行48小时的ML 188处理(0-30 μM),可有效抑制细胞中的 SARS-CoV 复制。在加入 ML188 之前,对 Vero E6 细胞进行模拟感染或1小时的SARS-CoV 感染,在感染后 48 小时,测定细胞活力,ML188 的 EC50 值为 12.9 ± 0.7 μM。
1. Jacobs J, et al. Discovery, synthesis, and structure-based optimization of a series of N-(tert-butyl)-2-(N-arylamido)-2-(pyridin-3-yl) acetamides (ML188) as potent noncovalent small molecule inhibitors of the severe acute respiratory syndrome coronavirus (SARS-CoV) 3CL protease. J Med Chem. 2013 Jan 24;56(2):534-46.
Storage | Store at -20°C |
M.Wt | 433.54 |
Cas No. | 1417700-13-0 |
Formula | C26H31N3O3 |
Solubility | ≥44.5 mg/mL in DMSO |
Chemical Name | (R)-N-(4-(tert-butyl)phenyl)-N-(2-(tert-butylamino)-2-oxo-1-(pyridin-3-yl)ethyl)furan-2-carboxamide |
SDF | Download SDF,smi |
Canonical SMILES | O=C([C@H](N(C(C1=CC=CO1)=O)C2=CC=C(C(C)(C)C)C=C2)C3=CN=CC=C3)NC(C)(C)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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