Manumycin A
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Manumycin A是一种抗生素，是一种有效的和选择性的法尼基转移酶抑制剂[1]。
法尼基转移酶通过增加一个法尼基到接近靶蛋白末端的半胱氨酸的巯基上，形成一个硫醚键，从而翻译后修饰蛋白。法尼基化使蛋白变成膜相关蛋白。
Manumycin A是一种有效的和选择性的ras法尼基转移酶抑制剂。在培养的MB细胞中，Manumycin A（≥10 µM）阻断p21 ras的法尼基化，诱导细胞凋亡[1]。在大鼠血管平滑肌细胞中，Manumycin A以剂量依赖的方式显著一致地抑制[3H]-胸苷掺入，抑制细胞生长。在Transwell侵袭实验中，manumycin A (50 ng/ml)在10 ng/ml的PDGF存在时抑制VSMC迁移[2]。
在有250个CUG重复的肌强直性营养不良1型（DM1）小鼠中，Manumycin A显著减少Clcn1外显子7A包含，纠正Clcn1的异常剪接[3]。
参考文献：
Wang W, Macaulay RJ. Apoptosis of medulloblastoma cells in vitro follows inhibition of farnesylation using manumycin A. Int J Cancer. 1999 Jul 30;82(3):430-4.
Kouchi H, Nakamura K, Fushimi K, et al. Manumycin A, inhibitor of ras farnesyltransferase, inhibits proliferation and migration of rat vascular smooth muscle cells. Biochem Biophys Res Commun. 1999 Nov 2;264(3):915-20.
Oana K, Oma Y, Suo S, et al. Manumycin A corrects aberrant splicing of Clcn1 in myotonic dystrophy type 1 (DM1) mice. Sci Rep. 2013;3:2142.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 550.64 |
| Cas No. | 52665-74-4 |
| Formula | C31H38N2O7 |
| Solubility | Soluble in DMSO |
| Chemical Name | (R,2Z,4E)-N-((1S,5S,6R)-5-hydroxy-5-((1E,3Z,5Z)-7-((2-hydroxy-5-oxocyclopent-1-en-1-yl)amino)-7-oxohepta-1,3,5-trien-1-yl)-2-oxo-7-oxabicyclo[4.1.0]hept-3-en-3-yl)-2,4,6-trimethyldeca-2,4-dienamide |
| SDF | Download SDF |
| Canonical SMILES | CCCC[C@H](/C=C(/C=C(C(NC1=C[C@@](/C=C/C=C\C=C/C(NC2=C(CCC2=O)O)=O)([C@@H]3O[C@@H]3C1=O)O)=O)/C)C)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
