Large T antigen - rhesus polyomavirus 560-568
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
大T抗原-恒河猴多瘤病毒560-568多肽序列为Ser-Glu-Phe-Leu-Leu-Glu-Lys-Arg-Ile。
T抗原对于病毒DNA复制、转录和病毒组装是必需的。另外，T抗原靶向多个细胞途径，包括调节细胞增殖、细胞死亡和炎症反应的途径。大T抗原通过与病毒来源的DNA复制结合，促进DNA合成，在调节病毒生命周期中发挥关键作用。作为多瘤病毒，可依赖于宿主细胞的细胞器进行复制，这个过程需要处于s期的宿主细胞才能起始复制过程。由于这个原因，大T抗原还调节细胞信号途径，通过结合许多胞内蛋白刺激细胞周期进展。
作为人类和动物的肿瘤病毒，多瘤病毒已被广泛研究，对癌变、DNA复制和蛋白质加工具有深入了解。肿瘤抑制分子p53作为胞内蛋白，与Simian vacuolating virus 40（SV40）产生的主要癌蛋白T抗原的结合。
Figure 1: Genome of the polyomaviruses
参考文献：
1. An P, Saenz Robeles MT, Pipas JM. “Large T antigens of polyomaviruses: amazing molecular machines”. Annu Rev Microbiol. 2012; 66:213-36.
2. White MK, Gordon J, Reiss K, et al. (December 2005). "Human polyomaviruses and brain tumors". Brain Research. Brain Research Reviews 50 (1): 69–85.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 1134.33 |
| Formula | C52H87N13O15 |
| Synonyms | H2N-Ser-Glu-Phe-Leu-Leu-Glu-Lys-Arg-Ile-OH |
| Solubility | ≥113.4 mg/mL in DMSO; insoluble in EtOH; ≥23.13 mg/mL in H2O |
| SDF | Download SDF |
| Canonical SMILES | N[C@H](C(N[C@@H](CCC(O)=O)C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CC(C)C)C(N[C@@H](CC(C)C)C(N[C@@H](CCC(O)=O)C(N[C@@H](CCCCN)C(N[C@@H](CCCNC(N)=N)C(N[C@@H]([C@@H](C)CC)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)CO |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
