Lamin fragment
![mRNA synthesis](/media/diy/images/page/figure1-mrna.png)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
![Tyramine Signal Amplification (TSA)](/media/diy/images/page/figure2-01.png)
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
![screening library](/media/diy/images/page/figure3-01.png)
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
![Cell Counting Kit-8 (CCK-8)](/media/diy/images/page/CCK-8.jpg)
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
![SYBR Safe DNA Gel Stain](/media/diy/images/page/SYBR Safe DNA Gel Stain.png)
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
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Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Lamin片段(核纤层蛋白片段)的多肽序列为Lys-Ala-Gly-Gln-Val-Val-Thr-Ile-Trp。
核纤层蛋白是V型中间丝,根据在细胞周期中的同源性序列、生物化学性质和细胞定位,可将其分为A型或B型,A型包括核纤层蛋白A和C,B型包括核纤层蛋白B1和B2。核纤层蛋白片段几乎涵盖了所有的α螺旋构象,由非α螺旋连接子分隔成多个α螺旋结构域,这些α螺旋结构域的氨基酸序列和长度高度保守。
核纤层蛋白是中间丝型的蛋白质,是核纤层的主要组成部分,核纤层是内核膜底层纤维状蛋白网络。核纤层蛋白也可以以弥漫性或斑点模式定位在细胞核内,在DNA复制、染色质组织、核孔复合体的空间排列、核生长以及核膜蛋白的锚固中发挥作用。
Figure 1: Lamin fragment
参考文献:
1. The Cell: A Molecular Approach, Cooper & Hausman. 5th Edition. Pg. 357
2. Ayelet Margalit, Sylvia Vlcek, Yozef Gruenbaum, Roland Foisner (2005). Breaking and Making of the Nuclear Envelope. Journal of Cellular Biochemistry 95, 454-465
3. Bruce Alberts, et al. Molecular Biology of the Cell (4th edition). Garland Science 676-677
4. Geoffrey M. Cooper, Robert E. Hausman. The Cell, A Molecular Approach (4th edition). Sinauer Associates 356-360
5. Goldman et al.(2002). "Nuclear lamins: building blocks of nuclear architecture". Genes and Development 16,533-547
6. Joanna M. Bridger, Nicole Foeger, Ian R. Kill, Harald Herrmann (2007). The Nuclear Lamina: both a structural framework and a platform for genome organization. FEBS Journal 274, 1354–1361
7. Nico Stuurman, Susanne Heins, Ueli Aebi (1998). Nuclear Lamins: Their Structure, Assembly and Interactions. Journal of Structural Biology 122, 42-46
8. Tripathi K, Muralikrishna B and Parnaik VK (2009) Differential dynamics and stability of lamin A rod domain mutants IJIB, 5(1), 1-8
9. Yozef Gruenbaum, Katherine L. Wilson, Amnon Harel, Michal Goldberg, Merav Cohen (2000). Nuclear Lamins – Structural Proteins with fundamental functions. Journal of Structural Biology129, 313-323
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 1001.18 |
Formula | C47H76N12O12 |
Synonyms | H2N-Lys-Ala-Gly-Gln-Val-Val-Thr-Ile-Trp-OH |
Solubility | ≥100.1 mg/mL in DMSO; insoluble in EtOH; ≥22 mg/mL in H2O |
SDF | Download SDF |
Canonical SMILES | NC(CCCCN)C(NC(C)C(NCC(NC(CCC(N)=O)C(NC(C(C)C)C(NC(C(C)C)C(NC(C(C)O)C(NC(C(C)CC)C(NC(CC1=CNC2=C1C=CC=C2)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
![Lamin fragment](http://www.apexbt.com//media/diy/images/struct/A1077.png)