KNK437
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
KNK437是亚苄基内酰胺化合物,抑制应激诱导的HSP(热休克蛋白)合成.
热休克蛋白(HSPs)是分子伴侣,在不同的细胞应激下其表达增加,如温度升高.它在调节蛋白质折叠中发挥至关重要的作用,与癌症和心血管疾病相关.
在人结肠癌细胞(COLO 320DM)中,KNK437抑制耐热的获得,以剂量依赖性方式诱导各种热休克蛋白.KNK437也会阻断亚砷酸钠诱发的耐热性获得[1].
KNK437在体内毒性较低.在移植SCC VII 细胞的C3H/He小鼠中,肿瘤中KNK437的浓度逐渐增加,腹膜内注射6小时后达到峰值.44℃高温处理10分钟,8小时后Hsp72被合成.在热刺激之前6小时KNK437给药可抑制其合成[2].
参考文献：
1. Yokota S, Kitahara M, Nagata K. Benzylidene lactam compound, KNK437, a novel inhibitor of acquisition of thermotolerance and heat shock protein induction in human colon carcinoma cells. Cancer Res. 2000 Jun 1;60(11):2942-8.
2. Koishi M, Yokota S, Mae T et al. The effects of KNK437, a novel inhibitor of heat shock protein synthesis, on the acquisition of thermotolerance in a murine transplantable tumor in vivo. Clin Cancer Res. 2001 Jan;7(1):215-9.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 245.23 |
| Cas No. | 218924-25-5 |
| Formula | C13H11NO4 |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥12.25 mg/mL in DMSO with ultrasonic |
| Chemical Name | (E)-3-(benzo[d][1,3]dioxol-5-ylmethylene)-2-oxopyrrolidine-1-carbaldehyde |
| SDF | Download SDF |
| Canonical SMILES | O=CN1C(/C(CC1)=C/C2=CC=C(OCO3)C3=C2)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
